High-resolution epitope mapping by AllerScan reveals relationships between IgE and IgG repertoires during peanut oral immunotherapy

Genghao Chen, Ellen L. Shrock, Mamie Z. Li, Jonathan M. Spergel, Kari C. Nadeau, Jacqueline A. Pongracic, Dale T. Umetsu, Rima Rachid, Andrew J. MacGinnitie, Wanda Phipatanakul, Lynda Schneider, Hans C. Oettgen, Stephen J. Elledge*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Peanut allergy can result in life-threatening reactions and is a major public health concern. Oral immunotherapy (OIT) induces desensitization to food allergens through administration of increasing amounts of allergen. To dissect peanut-specific immunoglobulin E (IgE) and IgG responses in subjects undergoing OIT, we have developed AllerScan, a method that leverages phage-display and next-generation sequencing to identify the epitope targets of peanut-specific antibodies. We observe a striking diversification and boosting of the peanut-specific IgG repertoire after OIT and a reduction in pre-existing IgE levels against individual epitopes. High-resolution epitope mapping reveals shared recognition of public epitopes in Ara h 1, 2, 3, and 7. In individual subjects, OIT-induced IgG specificities overlap extensively with IgE and exhibit strikingly similar antibody footprints, suggesting related clonal lineages or convergent evolution of peanut-specific IgE and IgG B cells. Individual differences in epitope recognition identified via AllerScan could inform safer and more effective personalized immunotherapy.

Original languageEnglish (US)
Article number100410
JournalCell Reports Medicine
Issue number10
StatePublished - Oct 19 2021


  • IgE
  • allergen immunotherapy
  • antibody
  • antibody repertoire
  • epitope mapping
  • food allergy
  • high-throughput sequencing
  • oral immunotherapy
  • peanut allergy
  • phage display

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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