High resolution MRI for non-invasive mouse lymph node mapping

Zhuoli Zhang, Daniel Procissi, Weiguo Li, Dong Hyun Kim, Kangan Li, Guohong Han, Yi Huan, Andrew C. Larson*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Mouse models are fundamental to the study and design of new techniques for the cancer diagnosis and treatment. The lymphatic system plays an active role in oncogenesis and metastatic disease progression. However, the in vivo identification of LNs in mice is challenging with conventional imaging modalities since the LN diameter in normal mice is 1-2. mm. Standard dissection techniques are challenging and can only provide endpoint data. Here, we describe high resolution MRI (HR-MRI) approaches for the non-invasive detection of mouse LNs in vivo. We compare in vivo non-invasive HR-MRI methods (without exogenous contrast injections) to the ex vivo dye injection methods for the identification of commonly studied LNs in both normal mice and a mouse model of pancreatic ductal adenocarcinoma (PDAC). We demonstrated the potential to use HR-MRI techniques as a non-invasive imaging assay for visualizing mouse LNs in vivo.

Original languageEnglish (US)
Pages (from-to)23-29
Number of pages7
JournalJournal of Immunological Methods
Volume400-401
Issue number1
DOIs
StatePublished - Jul 29 2013

Keywords

  • India ink
  • Lymph node
  • Lymphatic drainage
  • Magnetic resonance imaging
  • Mouse

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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