High-resolution three-dimensional micro-computed tomography detects bone loss and changes in trabecular architecture early: Comparison with DEXA and bone histomorphometry in a rat model of disuse osteoporosis

Odile Barou, David Valentin, Laurence Vico, Catherine Tirode, Alain Barbier, Christian Alexandre, Marie Hlne Lafage-Proust*

*Corresponding author for this work

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

Rationale and Objectives. The ability of three-dimensional micro-computed tomography (3D-μCT) to detect changes in a rat model of disuse osteoporosis was evaluated and compared with two reference techniques: dual x-ray absorptiometry (DEXA) for bone mass, and bone histomorphometry (BHM) for bone mass and trabecular micro-architecture. Methods. Forty-two rats were divided into controls or were hindlimb unloaded for 7, 13, and 23 days. DEXA bone mineral density measurements were performed on right tibiae. Then, after plastic embedding, bone volume (BV/TV) and trabecular (Tb)-derived parameters of trabecular bone architecture (Tb Th, thickness; Tb N, number) were measured with BHM. 3D-μCT measurements of BV/TV, Tb Th, and Tb N were carried out on left tibiae. Results. Unloaded rats lost bone in a time-dependent manner. DEXA and 3D-μCT detected bone loss earlier than BHM. The decreases in Tb Th and Tb N were observed at day 13 only with 3D-μCT (P < 0.05 and P < 0.01, respectively). All bone mass and architectural parameters measured with the three techniques correlated significantly (0.59, 0.89, P < 0.001), except Tb Th. Conclusions. 3D-μCT is a valid technique for bone mass and micro-architecture measurements in this rat model of disuse osteoporosis.

Original languageEnglish (US)
Pages (from-to)40-46
Number of pages7
JournalInvestigative radiology
Volume37
Issue number1
DOIs
StatePublished - Jan 1 2002

Keywords

  • Bone histology-unloading
  • Bone mineral density-immobilization
  • μCT

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'High-resolution three-dimensional micro-computed tomography detects bone loss and changes in trabecular architecture early: Comparison with DEXA and bone histomorphometry in a rat model of disuse osteoporosis'. Together they form a unique fingerprint.

  • Cite this