TY - JOUR
T1 - High Throughput and Highly Controllable Methods for In Vitro Intracellular Delivery
AU - Brooks, Justin
AU - Minnick, Grayson
AU - Mukherjee, Prithvijit
AU - Jaberi, Arian
AU - Chang, Lingqian
AU - Espinosa, Horacio D.
AU - Yang, Ruiguo
N1 - Funding Information:
R.Y. acknowledges the funding support from the NSF (award No. 1826135, No. 1936065), the NIH National Institutes of General Medical Sciences P20GM113126 (Nebraska Center for Integrated Biomolecular Communication), and P30GM127200 (Nebraska Center for Nanomedicine), the Nebraska Collaborative Initiative and Nebraska EPSCoR FIRST award. H.D.E. acknowledges the support of the NIH R21 Award Number GM132709‐01 and the National Cancer Institute of the National Institutes of Health (NIH) under Award Number U54CA199091.
Publisher Copyright:
© 2020 Wiley-VCH GmbH
PY - 2020/12/22
Y1 - 2020/12/22
N2 - In vitro and ex vivo intracellular delivery methods hold the key for releasing the full potential of tissue engineering, drug development, and many other applications. In recent years, there has been significant progress in the design and implementation of intracellular delivery systems capable of delivery at the same scale as viral transfection and bulk electroporation but offering fewer adverse outcomes. This review strives to examine a variety of methods for in vitro and ex vivo intracellular delivery such as flow-through microfluidics, engineered substrates, and automated probe-based systems from the perspective of throughput and control. Special attention is paid to a particularly promising method of electroporation using micro/nanochannel based porous substrates, which expose small patches of cell membrane to permeabilizing electric field. Porous substrate electroporation parameters discussed include system design, cells and cargos used, transfection efficiency and cell viability, and the electric field and its effects on molecular transport. The review concludes with discussion of potential new innovations which can arise from specific aspects of porous substrate-based electroporation platforms and high throughput, high control methods in general.
AB - In vitro and ex vivo intracellular delivery methods hold the key for releasing the full potential of tissue engineering, drug development, and many other applications. In recent years, there has been significant progress in the design and implementation of intracellular delivery systems capable of delivery at the same scale as viral transfection and bulk electroporation but offering fewer adverse outcomes. This review strives to examine a variety of methods for in vitro and ex vivo intracellular delivery such as flow-through microfluidics, engineered substrates, and automated probe-based systems from the perspective of throughput and control. Special attention is paid to a particularly promising method of electroporation using micro/nanochannel based porous substrates, which expose small patches of cell membrane to permeabilizing electric field. Porous substrate electroporation parameters discussed include system design, cells and cargos used, transfection efficiency and cell viability, and the electric field and its effects on molecular transport. The review concludes with discussion of potential new innovations which can arise from specific aspects of porous substrate-based electroporation platforms and high throughput, high control methods in general.
KW - electroporation
KW - intracellular delivery
KW - localized cell electroporation
KW - porous substrates
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U2 - 10.1002/smll.202004917
DO - 10.1002/smll.202004917
M3 - Review article
C2 - 33241661
AN - SCOPUS:85096663958
VL - 16
JO - Small
JF - Small
SN - 1613-6810
IS - 51
M1 - 2004917
ER -