High-throughput screening of small molecule libraries using SAMDI mass spectrometry

Zachary A. Gurard-Levin, Michael D. Scholle, Adam H. Eisenberg, Milan Mrksich*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

84 Scopus citations


High-throughput screening is a common strategy used to identify compounds that modulate biochemical activities, but many approaches depend on cumbersome fluorescent reporters or antibodies and often produce false-positive hits. The development of "label-free" assays addresses many of these limitations, but current approaches still lack the throughput needed for applications in drug discovery. This paper describes a high-throughput, label-free assay that combines self-assembled monolayers with mass spectrometry, in a technique called SAMDI, as a tool for screening libraries of 100 000 compounds in one day. This method is fast, has high discrimination, and is amenable to a broad range of chemical and biological applications.

Original languageEnglish (US)
Pages (from-to)347-350
Number of pages4
JournalACS Combinatorial Science
Issue number4
StatePublished - Jul 11 2011

ASJC Scopus subject areas

  • General Chemistry


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