Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes

Paul W. Burridge, Elias T. Zambidis*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

24 Scopus citations

Abstract

Human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes are a novel source of cells for patient-specific cardiotoxicity drug testing, drug discovery, disease modeling, and regenerative medicine. We describe a versatile and cost-effective protocol for in vitro cardiac differentiation that is effective for a wide variety of hiPSC and human embryonic stem cell (hESC) lines. This highly optimized protocol produces contracting human embryoid bodies (hEB) with a near total efficiency of 94.7 ± 2.4% in less than 9 days, and minimizes the variability in cardiac differentiation commonly observed between various hiPSC and hESC lines. The contracting hEB derived using these methods contain high percentages of pure functional cardiomyocytes, highly reproducible electrophysiological profiles, and pharmacologic responsiveness to known cardioactive drugs.

Original languageEnglish (US)
Title of host publicationPluripotent Stem Cells
Subtitle of host publicationMethods and Protocols
EditorsUma Lakshmipathy, Mohan Vemuri
Pages149-161
Number of pages13
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume997
ISSN (Print)1064-3745

Keywords

  • Cardiac
  • Cardiomyocyte
  • Differentiation
  • Forced aggregation
  • Heart
  • Human embryonic stem cell
  • Induced pluripotent stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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