Highly restricted expression at the ectoderm-endoderm boundary of PIHbox 9, a sea urchin homeobox gene related to the human HB9 gene

Daniela Bellomonte, Maria Di Bernardo, Roberta Russo, Giuliana Caronia, Giovanni Spinelli*

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Characterisation of a sea urchin (P. lividus) homeobox gene PIHbox 9 is reported. The homeodomain of PIHbox9 is 95% identical to the homeodomain of the human HB9 gene, indicating that the two genes are highly related. Temporal expression analysis during sea urchin embryogenesis showed an absence of transcripts at early cleavage stages. At late gastrula stage, transcripts were barely detectable and reached the highest abundance at prism/early pluteus stages. By whole mount in situ hybridisation we observed a highly restricted expression in a few cells of the ectoderm-endoderm boundary of embryos at the prism stage. At pluteus stages, expression of PIHbox 9 was confined around the anus.

Original languageEnglish (US)
Pages (from-to)185-188
Number of pages4
JournalMechanisms of Development
Volume74
Issue number1-2
DOIs
StatePublished - Jun 1 1998

Keywords

  • Ectoderm-endoderm boundary
  • Human HB9 related
  • PIHbox 9 homeogene
  • Paracentrotus lividus
  • Sea urchin embryogenesis
  • Whole mount in situ hybridisation

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

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