Highly specific electrochemical analysis of cancer cells using multi-nanoparticle labeling

Ying Wan, Yi Ge Zhou, Mahla Poudineh, Tina Saberi Safaei, Reza M. Mohamadi, Edward H. Sargent, Shana O. Kelley

Research output: Contribution to journalArticlepeer-review

79 Scopus citations

Abstract

Circulating tumor cells (CTCs) can be collected noninvasively and provide a wealth of information about tumor phenotype. For this reason, their specific and sensitive detection is of intense interest. Herein, we report a new, chip-based strategy for the automated analysis of cancer cells. The nanoparticle-based, multi-marker approach exploits the direct electrochemical oxidation of metal nanoparticles (MNPs) to report on the presence of specific surface markers. The electrochemical assay allows simultaneous detection of multiple different biomarkers on the surfaces of cancer cells, enabling discrimination between cancer cells and normal blood cells. Through multiplexing, it further enables differentiation among distinct cancer cell types. We showcase the technology by demonstrating the detection of cancer cells spiked into blood samples.

Original languageEnglish (US)
Pages (from-to)13145-13149
Number of pages5
JournalAngewandte Chemie - International Edition
Volume53
Issue number48
DOIs
StatePublished - Nov 24 2014
Externally publishedYes

Keywords

  • Antibodies
  • Cancer cells
  • Electrochemistry
  • Metal nanoparticles
  • Surface markers

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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