Hippocampal magnetization transfer ratio at 3T: Validation of automated postprocessing and comparison of quantification metrics

Shawn Sidharthan, Ryan Hutten, Christopher Glielmi, Hongyan Du, Fiona Malone, Ann B. Ragin, Robert R. Edelman, Ying Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


BACKGROUND: To investigate the reliability of a novel magnetization transfer ratio (MTR) postprocessing technique for the hippocampus using histogram analysis, and compare results to more established volumetric measurements. This study is conducted in healthy volunteers as a precursor to future applications regarding progressive neurologic diseases, such as Alzheimer's disease. METHODS: Eight healthy subjects were scanned twice with interval of 1 week using quantitative magnetic resonance imaging (MRI). Automated pixel-wise analysis was performed for the hippocampal regions of each patient. Reliability was assessed using intraclass correlation coefficients (ICCs), coefficients of variation (COVs), and instrumental standard deviation (ISD). RESULTS: Reliable metrics were 25th percentile, median, 75th percentile, peak location, and mean approach (ranges: ICC = 93-96, COV = 2.71-3.88%, ISD .78-1.01). Histogram peak height had ICC below .7, and a COV above 10%. Volumetric measurements had (ICC = .95-97, COV = 1.43-3.39%). CONCLUSION: Excellent scan-rescan reproducibility (ICC > .9, COV < 10%) was observed for specific MTR histogram metrics and the mean MTR approach. These results are comparable to the volumetric approach. Future studies can examine the possibility that MTR changes precede morphological changes as this study suggests that both MTR and volumetric measurements of the hippocampus can be used as reliable imaging tools.

Original languageEnglish (US)
Pages (from-to)484-490
Number of pages7
JournalJournal of Neuroimaging
Issue number3
StatePublished - Jul 2013


  • 3T
  • Histogram analysis
  • Magnetization transfer imaging
  • Magnetization transfer ratio
  • Normal-control analysis
  • Reproducibility

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Clinical Neurology


Dive into the research topics of 'Hippocampal magnetization transfer ratio at 3T: Validation of automated postprocessing and comparison of quantification metrics'. Together they form a unique fingerprint.

Cite this