Hippocampal Pyramidal Neurons Comprise Two Distinct Cell Types that Are Countermodulated by Metabotropic Receptors

Austin R. Graves; Shannon J. Moore; Erik B. Bloss; Brett D. Mensh; William L. Kath; Nelson Spruston

doi:10.1016/j.neuron.2012.09.036

Hippocampal Pyramidal Neurons Comprise Two Distinct Cell Types that Are Countermodulated by Metabotropic Receptors

Austin R. Graves, Shannon J. Moore, Erik B. Bloss, Brett D. Mensh, William L. Kath, Nelson Spruston^*

^*Corresponding author for this work

Engineering Sciences and Applied Mathematics

Research output: Contribution to journal › Article › peer-review

154 Scopus citations

Abstract

Relating the function of neuronal cell types to information processing and behavior is a central goal of neuroscience. In the hippocampus, pyramidal cells in CA1 and the subiculum process sensory and motor cues to form a cognitive map encoding spatial, contextual, and emotional information, which they transmit throughout the brain. Do these cells constitute a single class or are there multiple cell types with specialized functions? Using unbiased cluster analysis, we show that there are two morphologically and electrophysiologically distinct principal cell types that carry hippocampal output. We show further that these two cell types are inversely modulated by the synergistic action of glutamate and acetylcholine acting on metabotropic receptors that are central to hippocampal function. Combined with prior connectivity studies, our results support a model of hippocampal processing in which the two pyramidal cell types are predominantly segregated into two parallel pathways that process distinct modalities of information.

Original language	English (US)
Pages (from-to)	776-789
Number of pages	14
Journal	Neuron
Volume	76
Issue number	4
DOIs	https://doi.org/10.1016/j.neuron.2012.09.036
State	Published - Nov 21 2012

Funding

The authors would like to members of the Spruston laboratory for helpful discussions and Adam Hantman for reagents. A.R.G. was supported by F31 NS067758 and A.R.G. and S.J.M. were supported by T32 MH067564. The work was also supported by NIH RO1 NS35180, NS-046064, NS-077601, and the Howard Hughes Medical Institute. A.R.G., S.J.M., and N.S. designed the experiments. A.R.G. and S.J.M. collected the electrophysiological data, A.R.G. collected the morphological data, and A.R.G. and E.B.B. performed the immunostaining and imaging. A.R.G. analyzed the data, with input from N.S. and help from W.L.K. to perform the cluster and principal component analyses. A.R.G., B.B.M., and N.S. wrote the manuscript, with input from the other authors.

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neuron.2012.09.036

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title = "Hippocampal Pyramidal Neurons Comprise Two Distinct Cell Types that Are Countermodulated by Metabotropic Receptors",

abstract = "Relating the function of neuronal cell types to information processing and behavior is a central goal of neuroscience. In the hippocampus, pyramidal cells in CA1 and the subiculum process sensory and motor cues to form a cognitive map encoding spatial, contextual, and emotional information, which they transmit throughout the brain. Do these cells constitute a single class or are there multiple cell types with specialized functions? Using unbiased cluster analysis, we show that there are two morphologically and electrophysiologically distinct principal cell types that carry hippocampal output. We show further that these two cell types are inversely modulated by the synergistic action of glutamate and acetylcholine acting on metabotropic receptors that are central to hippocampal function. Combined with prior connectivity studies, our results support a model of hippocampal processing in which the two pyramidal cell types are predominantly segregated into two parallel pathways that process distinct modalities of information.",

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AU - Spruston, Nelson

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AB - Relating the function of neuronal cell types to information processing and behavior is a central goal of neuroscience. In the hippocampus, pyramidal cells in CA1 and the subiculum process sensory and motor cues to form a cognitive map encoding spatial, contextual, and emotional information, which they transmit throughout the brain. Do these cells constitute a single class or are there multiple cell types with specialized functions? Using unbiased cluster analysis, we show that there are two morphologically and electrophysiologically distinct principal cell types that carry hippocampal output. We show further that these two cell types are inversely modulated by the synergistic action of glutamate and acetylcholine acting on metabotropic receptors that are central to hippocampal function. Combined with prior connectivity studies, our results support a model of hippocampal processing in which the two pyramidal cell types are predominantly segregated into two parallel pathways that process distinct modalities of information.

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Hippocampal Pyramidal Neurons Comprise Two Distinct Cell Types that Are Countermodulated by Metabotropic Receptors

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