Abstract
Background: Reproductive aging is a robust phenotype that occurs in all females and is characterized by a significant reduction in gamete quantity and quality, which can have negative consequences on both endocrine function and fertility. Age-associated differences in the oocyte, follicle, and ovary have been well-documented, but how the broader environment changes with age is less well understood. Fat is one of the largest organs in the body, and peri-gonadal adipose tissue surrounds the rodent ovary and comprises a local ovarian environment. The goal of this study was to characterize how peri-ovarian adipose tissue changes with advanced reproductive age. Methods: We isolated peri-gonadal adipose tissue from two cohorts of CB6F1 mice: reproductively young (6-12 weeks) and reproductively old (14-17 months). A comparative histological analysis was performed to evaluate adipocyte architecture. We then extracted lipids from the tissue and performed multiple reaction monitoring (MRM)-profiling, a mass spectrometry-based method of metabolite profiling, to compare the lipid profiles of peri-gonadal adipose tissue in these age cohorts. Results: We found that advanced reproductive age was associated with adipocyte hypertrophy and a corresponding decrease in the number of adipocytes per area. Of the 10 lipid classes examined, triacylglycerols (TAGs) had significantly different profiles between young and old cohorts, despite quantitative analysis revealing a decrease in the total amount of TAGs per weight of peri-gonadal adipose tissue with age. Conclusions: These findings pinpoint age-associated physiological changes in peri-gonadal adipose tissue with respect to adipocyte morphology and lipid profiles and lay the foundation for future studies to examine how these alterations may influence both adipocyte and ovarian function.
Original language | English (US) |
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Article number | 46 |
Journal | Reproductive Biology and Endocrinology |
Volume | 17 |
Issue number | 1 |
DOIs | |
State | Published - Jun 12 2019 |
Funding
This research was supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD093726 to F.E.D and M.T.P.) and startup funds from the Department of Obstetrics and Gynecology (to F.E.D).
Keywords
- Adipose
- Aging
- Ovary
- Peri-gonadal
- Triacylglycerol
ASJC Scopus subject areas
- Reproductive Medicine
- Endocrinology
- Obstetrics and Gynecology
- Developmental Biology
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Histologic analysis and lipid profiling reveal reproductive age-associated changes in peri-ovarian adipose tissue
Dipali, S. S. (Creator), Ferreira, C. R. (Creator), Zhou, L. T. (Contributor), Pritchard, M. T. (Creator) & Duncan, F. E. (Creator), figshare, 2019
DOI: 10.6084/m9.figshare.c.4539929, https://springernature.figshare.com/collections/Histologic_analysis_and_lipid_profiling_reveal_reproductive_age-associated_changes_in_peri-ovarian_adipose_tissue/4539929
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