Histologic and molecular profile of pediatric insulinomas: Evidence of a paternal parent-of-origin effect

Tricia R. Bhatti*, Karthik Ganapathy, Alison R. Huppmann, Laura Conlin, Kara E. Boodhansingh, Courtney MacMullen, Susan Becker, Linda M. Ernst, N. Scott Adzick, Eduardo D. Ruchelli, Arupa Ganguly, Charles A. Stanley

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Context: Acquired insulinomas are rare causes of hyperinsulinemic hypoglycemia in children and are much less common than focal lesions of congenital hyperinsulinism. The latter are known to be associated with isodisomy for paternally transmitted ATP-sensitive potassium channel mutations on 11p15; however, the molecular basis for pediatric insulinomas is not well characterized. Objective: The purpose of this study was to characterize the histopathological and molecular defects in a large group of 12 pediatric insulinomas seen at The Children's Hospital of Philadelphia. Results: Twelve children with insulinomas were seen between 1971 and 2013, compared to 201 cases with focal congenital hyperinsulinism seen between 1997 and 2014. The age of insulinoma patients ranged from 4-16 years at the time of surgery. Features of MEN1 syndrome were present in five of the 12, including four cases with heterozygous mutations of MEN1 on 11q. Immunohistochemical analysis revealed nuclear loss of p57 staining consistent with loss of the maternal 11p15 allele in 11 of the 12 insulinomas, including all five MEN1-associated tumors. Imbalance of the paternal 11p allele was confirmed by single nucleotide polymorphism genotyping and methylation assays of the 11p imprinting control loci in four of five MEN1-associated tumors and six of seven sporadic insulinomas. In addition, single nucleotide polymorphism genotyping revealed extensive tumor aneuploidy beyond chromosome 11. Conclusions: These data indicate thatMEN1mutations aremorecommonin insulinomas in children than in adults. Aneuploidy of chromosome 11 and other chromosomes is common in both MEN1 andnon-MEN1insulinomas.Thenovel observation of a paternal parent-of-origin effect in allMEN1 and most non-MEN1 tumors suggests a critical role for imprinted growth-regulatory genes in the 11p region in the genesis of β-cell endocrine tumors in children.

Original languageEnglish (US)
Pages (from-to)914-922
Number of pages9
JournalJournal of Clinical Endocrinology and Metabolism
Volume101
Issue number3
DOIs
StatePublished - Mar 2016

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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    Bhatti, T. R., Ganapathy, K., Huppmann, A. R., Conlin, L., Boodhansingh, K. E., MacMullen, C., Becker, S., Ernst, L. M., Scott Adzick, N., Ruchelli, E. D., Ganguly, A., & Stanley, C. A. (2016). Histologic and molecular profile of pediatric insulinomas: Evidence of a paternal parent-of-origin effect. Journal of Clinical Endocrinology and Metabolism, 101(3), 914-922. https://doi.org/10.1210/jc.2015-2914