TY - JOUR
T1 - Histone H2B Monoubiquitination Functions Cooperatively with FACT to Regulate Elongation by RNA Polymerase II
AU - Pavri, Rushad
AU - Zhu, Bing
AU - Li, Guohong
AU - Trojer, Patrick
AU - Mandal, Subhrangsu
AU - Shilatifard, Ali
AU - Reinberg, Danny
N1 - Funding Information:
We thank Drs. J. Lis, L. Vales, and F. Jeffrey Dilworth for helpful comments and suggestions. This work was supported by a grant from the National Institutes of Health (GM37120) and the Howard Hughes Medical Institute to D.R.
PY - 2006/5/19
Y1 - 2006/5/19
N2 - Over the past years, a large number of histone posttranslational modifications have been described, some of which function to attain a repressed chromatin structure, while others facilitate activation by allowing access of regulators to DNA. Histone H2B monoubiquitination is a mark associated with transcriptional activity. Using a highly reconstituted chromatin-transcription system incorporating the inducible RARβ2 promoter, we find that the establishment of H2B monoubiquitination by RNF20/40 and UbcH6 is dependent on the transcription elongation regulator complex PAF, the histone chaperone FACT, and transcription. H2B monoubiquitination facilitates FACT function, thereby stimulating transcript elongation and the generation of longer transcripts. These in vitro analyses and corroborating in vivo experiments demonstrate that elongation by RNA polymerase II through the nucleosomal barrier is minimally dependent upon (1) FACT and (2) the recruitment of PAF and the H2B monoubiquitination machinery.
AB - Over the past years, a large number of histone posttranslational modifications have been described, some of which function to attain a repressed chromatin structure, while others facilitate activation by allowing access of regulators to DNA. Histone H2B monoubiquitination is a mark associated with transcriptional activity. Using a highly reconstituted chromatin-transcription system incorporating the inducible RARβ2 promoter, we find that the establishment of H2B monoubiquitination by RNF20/40 and UbcH6 is dependent on the transcription elongation regulator complex PAF, the histone chaperone FACT, and transcription. H2B monoubiquitination facilitates FACT function, thereby stimulating transcript elongation and the generation of longer transcripts. These in vitro analyses and corroborating in vivo experiments demonstrate that elongation by RNA polymerase II through the nucleosomal barrier is minimally dependent upon (1) FACT and (2) the recruitment of PAF and the H2B monoubiquitination machinery.
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U2 - 10.1016/j.cell.2006.04.029
DO - 10.1016/j.cell.2006.04.029
M3 - Article
C2 - 16713563
AN - SCOPUS:33646691283
VL - 125
SP - 703
EP - 717
JO - Cell
JF - Cell
SN - 0092-8674
IS - 4
ER -