HIV expression is induced by dying cells

Gayle E. Woloschak; Steven Schreck; John Panozzo; Chin Mei Chang-Liu; Claudia R. Libertin

doi:10.1016/S0167-4781(96)00184-4

HIV expression is induced by dying cells

Gayle E. Woloschak^*, Steven Schreck, John Panozzo, Chin Mei Chang-Liu, Claudia R. Libertin

^*Corresponding author for this work

Radiation Oncology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. γ rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that γ-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. Doses which caused over 99% cell killing induced HIV-LTR transcription maximally, demonstrating that cells that will go on to die by 14 days are the cells expressing HIV-LTR-CAT.

Original language	English (US)
Pages (from-to)	105-110
Number of pages	6
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1351
Issue number	1-2
DOIs	https://doi.org/10.1016/S0167-4781(96)00184-4
State	Published - Mar 20 1997

Keywords

Apoptosis
DNA damage
HIV-LTR expression
Radiation
Stress

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0167-4781(96)00184-4

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title = "HIV expression is induced by dying cells",

abstract = "Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. γ rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that γ-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. Doses which caused over 99% cell killing induced HIV-LTR transcription maximally, demonstrating that cells that will go on to die by 14 days are the cells expressing HIV-LTR-CAT.",

keywords = "Apoptosis, DNA damage, HIV-LTR expression, Radiation, Stress",

author = "Woloschak, {Gayle E.} and Steven Schreck and John Panozzo and Chang-Liu, {Chin Mei} and Libertin, {Claudia R.}",

note = "Funding Information: The authors wish to thank Kay Bexson for excellent secretarial assistance and Gordon Holmblad for assistance in irradiations. This work was supported by the U.S. Department of Energy, Office of Health and Environmental Research, under Contract W-31-109-ENG-38 and NIEHS grant #ES07141.",

year = "1997",

month = mar,

day = "20",

doi = "10.1016/S0167-4781(96)00184-4",

language = "English (US)",

volume = "1351",

pages = "105--110",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

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TY - JOUR

T1 - HIV expression is induced by dying cells

AU - Woloschak, Gayle E.

AU - Schreck, Steven

AU - Panozzo, John

AU - Chang-Liu, Chin Mei

AU - Libertin, Claudia R.

N1 - Funding Information: The authors wish to thank Kay Bexson for excellent secretarial assistance and Gordon Holmblad for assistance in irradiations. This work was supported by the U.S. Department of Energy, Office of Health and Environmental Research, under Contract W-31-109-ENG-38 and NIEHS grant #ES07141.

PY - 1997/3/20

Y1 - 1997/3/20

N2 - Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. γ rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that γ-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. Doses which caused over 99% cell killing induced HIV-LTR transcription maximally, demonstrating that cells that will go on to die by 14 days are the cells expressing HIV-LTR-CAT.

AB - Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. γ rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that γ-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. Doses which caused over 99% cell killing induced HIV-LTR transcription maximally, demonstrating that cells that will go on to die by 14 days are the cells expressing HIV-LTR-CAT.

KW - Apoptosis

KW - DNA damage

KW - HIV-LTR expression

KW - Radiation

KW - Stress

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DO - 10.1016/S0167-4781(96)00184-4

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

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ER -

HIV expression is induced by dying cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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