HIV expression is induced by dying cells

Gayle E. Woloschak*, Steven Schreck, John Panozzo, Chin Mei Chang-Liu, Claudia R. Libertin

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. γ rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that γ-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. Doses which caused over 99% cell killing induced HIV-LTR transcription maximally, demonstrating that cells that will go on to die by 14 days are the cells expressing HIV-LTR-CAT.

Original languageEnglish (US)
Pages (from-to)105-110
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1351
Issue number1-2
DOIs
StatePublished - Mar 20 1997

Keywords

  • Apoptosis
  • DNA damage
  • HIV-LTR expression
  • Radiation
  • Stress

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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