@article{c853871027604c31a92f1053193fb533,
title = "Hlaallele Detection Using Molecular Techniques",
abstract = "There are now many molecular biological techniques available to define HLA class I and class II alleles. Some of these are also applicable to other human polymorphic genes, in particular to those non-HLA genes encoded within the Mhc. The range of techniques available allows laboratories to choose those most suited to their purpose. The routine laboratory supporting solid organ transplants will need to type large numbers of potential recipients over a period of time, probably using PCR-SSOP while donors will be typed singly and rapidly using PCR-SSP with HLA allele compatibility determined by heteroduplex analysis. Laboratories supporting bone marrow transplantation, where time is less pressing, can choose from the whole range of techniques to determine accurately donor recipient Mhc compatibility. For disease studies, techniques defining precise HLA allele sequence polymorphisms are needed and high sample numbers have to be accommodated. When an association is established allele sequencing has to be used. In the near future, the precise role of HLA alleles in transplantation and disease susceptibility is likely to be established unambiguously.",
keywords = "HLA and disease, HLA typing, Histocompatibility, PCR",
author = "Kay Poulton and Paul Sinnott and Damini Jawaheer and Bill Ollier and Kay Poulton and Wendy Thomson",
note = "Funding Information: Dyer Philip A. 1 Jawaheeri Damini 2 Allier Bill 2 Poulton Kay 1, 2 Sinnott Paul 1 Thomson Wendy 2 NW Regiollal Tissue Typing Laboratory St. Mary{\textquoteright}s Hospital Manchester UK ARC Epidemiology Research Unit University of Manchester Manchester UK 1993 28 5 2013 11 4 145 160 5 8 1993 1993 Copyright {\textcopyright} 1993 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. There are now many molecular biological techniques available to define HLA class I and class II alleles. Some of these are also applicable to other human polymorphic genes, in particular to those non-HLA genes encoded within the Mhc. The range of techniques available allows laboratories to choose those most suited to their purpose. The routine laboratory supporting solid organ transplants will need to type large numbers of potential recipients over a period of time, probably using PCR-SSOP while donors will be typed singly and rapidly using PCR-SSP with HLA allele compatibility determined by heteroduplex analysis. Laboratories supporting bone marrow transplantation, where time is less pressing, can choose from the whole range of techniques to determine accurately donor recipient Mhc compatibility. For disease studies, techniques defining precise HLA allele sequence polymorphisms are needed and high sample numbers have to be accommodated. When an association is established allele sequencing has to be used. In the near future, the precise role of HLA alleles in transplantation and disease susceptibility is likely to be established unambiguously. HLA typing PCR HLA and disease Histocompatibility Arthritis and Rheumatism Research Council NW Regional Health Authority National Kidney Research Fund Central Manchester Hospitals Trust ",
year = "1993",
doi = "10.1155/1993/215379",
language = "English (US)",
volume = "11",
pages = "145--160",
journal = "Disease Markers",
issn = "0278-0240",
publisher = "IOS Press",
number = "4",
}
