Hsp70 and Hsc70 are preferentially expressed in differentiated epithelial cells in normal human endometrium and ectocervix

Allison B. Mangurten, Kevin R. Brader, Benjamin M. Loos, Edward Lee, Ana I. Quiroga, Julie Bathori, John R. Lurain, Andrei Laszlo, Benette Phillips*

*Corresponding author for this work

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Two highly related 70K heat shock proteins, encoded by the hsc70 and hsp70 genes, are located in the nucleocytoplasmic compartment of mammalian cells. In contrast to rodent cell lines, which express Hsp70 only when stressed, many human cell lines constitutively express Hsp70. The degree to which this reflects constitutive expression of Hsp70 in normal human tissues has not been extensively examined. In this study, we show by immunoblotting that human Hsp70 is constitutively expressed in the ovary, cervix, and endometrium and, by immunohistochemical analysis using Hsp70- and Hsc70-specific antibodies, that Hsp70 and Hsc70 are expressed in distinctive and predominantly overlapping patterns in the cervix and endometrium. In these two tissues, the highest levels of both proteins are seen in differentiated, non-proliferating epithelial cells, which is surprising in light of previous studies suggesting growth stimulation of hsp70 gene expression. These observations suggest the possibility that in certain human tissues, basal expression of the hsp70 and hsc70 genes is coregulated.

Original languageEnglish (US)
Pages (from-to)168-174
Number of pages7
JournalCell Stress and Chaperones
Volume2
Issue number3
DOIs
StatePublished - Sep 1 1997

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Hsp70 and Hsc70 are preferentially expressed in differentiated epithelial cells in normal human endometrium and ectocervix'. Together they form a unique fingerprint.

  • Cite this