TY - JOUR
T1 - HSP70-related 65 kDa protein of beet yellows closterovirus is a microtubule-binding protein
AU - Karasev, Alexander V.
AU - Kashina, Anna S.
AU - Gelfand, Vladimir I.
AU - Dolja, Valerian V.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1992/6/8
Y1 - 1992/6/8
N2 - Beet yellows virus (BYV) genome encodes a 65 kDa protein homologous to the HSP70 family of cellular heat-shock proteins (Agranovsky, A.A., Boyko, V.P., Karasev, A.V., Koonin, E.V. and Dolja, V.V. (1991) J. Mol. Biol. 217, 603-610). The respective gene was cloned and expressed in vitro yielding a product of the expected size (p65). This product was found to bind to the purified microtubules with a binding constant of 4 × 10-7 M. The binding of p65 was stimulated if ATP presented in the translation mixture was hydrolyzed by apyrase. Removal of the short C-terminal domains of α- and β-tubulin by subtilisin digestion abolished the binding, demonstrating its specificity. The possible role of p65 association with microtubules in the movement of virus within and/or between plant cells is proposed.
AB - Beet yellows virus (BYV) genome encodes a 65 kDa protein homologous to the HSP70 family of cellular heat-shock proteins (Agranovsky, A.A., Boyko, V.P., Karasev, A.V., Koonin, E.V. and Dolja, V.V. (1991) J. Mol. Biol. 217, 603-610). The respective gene was cloned and expressed in vitro yielding a product of the expected size (p65). This product was found to bind to the purified microtubules with a binding constant of 4 × 10-7 M. The binding of p65 was stimulated if ATP presented in the translation mixture was hydrolyzed by apyrase. Removal of the short C-terminal domains of α- and β-tubulin by subtilisin digestion abolished the binding, demonstrating its specificity. The possible role of p65 association with microtubules in the movement of virus within and/or between plant cells is proposed.
KW - Cell-to-cell movement
KW - Microtubule-associated protein
KW - Plant virus
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U2 - 10.1016/0014-5793(92)80578-5
DO - 10.1016/0014-5793(92)80578-5
M3 - Article
C2 - 1618294
AN - SCOPUS:0026653019
VL - 304
SP - 12
EP - 14
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -