HSP70-related 65 kDa protein of beet yellows closterovirus is a microtubule-binding protein

Alexander V. Karasev, Anna S. Kashina, Vladimir I. Gelfand, Valerian V. Dolja*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Beet yellows virus (BYV) genome encodes a 65 kDa protein homologous to the HSP70 family of cellular heat-shock proteins (Agranovsky, A.A., Boyko, V.P., Karasev, A.V., Koonin, E.V. and Dolja, V.V. (1991) J. Mol. Biol. 217, 603-610). The respective gene was cloned and expressed in vitro yielding a product of the expected size (p65). This product was found to bind to the purified microtubules with a binding constant of 4 × 10-7 M. The binding of p65 was stimulated if ATP presented in the translation mixture was hydrolyzed by apyrase. Removal of the short C-terminal domains of α- and β-tubulin by subtilisin digestion abolished the binding, demonstrating its specificity. The possible role of p65 association with microtubules in the movement of virus within and/or between plant cells is proposed.

Original languageEnglish (US)
Pages (from-to)12-14
Number of pages3
JournalFEBS Letters
Volume304
Issue number1
DOIs
StatePublished - Jun 8 1992

Keywords

  • Cell-to-cell movement
  • Microtubule-associated protein
  • Plant virus

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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