Abstract
Human myosin heavy chains are encoded by a multigene family consisting of at least 10 members. A gene-specific oligonucleotide has been used to isolate the human beta myosin heavy chain gene from a group of twelve nonoverlapping genomic clones. We have shown that this gene (which is expressed in both cardiac and skeletal muscle) is located 3.6kb upstream of the alpha cardiac myosin gene. We find that DNA sequences located upstream of rat and human alpha cardiac myosin heavy chain genes are very homologous over a 300bp region. Analogous regions of two other myosin genes expressed in different muscles (cardiac and skeletal) show no such homology to each other. While a human skeletal muscle myosin heavy chain gene cluster is located on chromosome 17, we show that the beta and alpha human cardiac myosin heavy chain genes are located on chromosome 14.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5443-5459 |
| Number of pages | 17 |
| Journal | Nucleic acids research |
| Volume | 15 |
| Issue number | 13 |
| DOIs | |
| State | Published - Jul 10 1987 |
Funding
We would like to thank R. Schwartz for help with the restriction map. We would like to acknowledge the excellent secretarial assistance of D. Jackson and L. Vitagliano. This work was supported by grants from: AHA to L.L., NIH (GM29090 to L.L.; GM20454, HD05196 to T.S.), ACS CD62 to T.S. L.L. is an American Heart Established Investigator. E.M. is supported by M.S.T.P. Grant, 5T32 GM7288-12.
ASJC Scopus subject areas
- Genetics