Human CD34+ stem cells express the hiwi gene, a human homologue of the Drosophila gene piwi

Arun K. Sharma, Mary C. Nelson, John E. Brandt, Maija Wessman, Nadim Mahmud, Kevin P. Weller, Ronald Hoffman*

*Corresponding author for this work

Research output: Contribution to journalArticle

145 Scopus citations

Abstract

Hematopoietic stem cells (HSCs) are characterized by their dual abilities to undergo differentiation into multiple hematopoietic cell lineages or to undergo self-renewal. The molecular basis of these properties remains poorly understood. Recently the piwi gene was found in the embryonic germline stem cells (GSCs) of Drosophila melanogaster and has been shown to be important in GSC self-renewal. This study demonstrated that hiwi, a novel human homologue of piwi, is also present in human CD34+ hematopoietic progenitor cells but not in more differ entiated cell populations. Placing CD34+ cells into culture conditions that supported differentiation and rapid exit from the stem cell compartment resulted in a loss of hiwi expression by day 5 of a 14-day culture period. Expression of the hiwi gene was detected in many developing fetal and adult tissues. By means of 5′ RACE cloning methodology, a novel putative full-length hiwi complementary DNA was cloned from human CD34+ marrow cells. At the amino acid level, the human HIWI protein was 52% homologous to the Drosophila protein. The transient expression of hiwi in the human leukomia cell line KG1 resulted in a dramatic reduction in cellular proliferation. Over-expression of hiwi led to programmed cell death of KG1 cells as demonstrated by the Annexin V assay system. These studies suggest that hiwi maybe an important negative developmental regulator, which, in part, underlies the unique biologic properties associated with hematopoietic stem and progenitor cells.

Original languageEnglish (US)
Pages (from-to)426-434
Number of pages9
JournalBlood
Volume97
Issue number2
DOIs
StatePublished - Jan 15 2001

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Sharma, A. K., Nelson, M. C., Brandt, J. E., Wessman, M., Mahmud, N., Weller, K. P., & Hoffman, R. (2001). Human CD34+ stem cells express the hiwi gene, a human homologue of the Drosophila gene piwi. Blood, 97(2), 426-434. https://doi.org/10.1182/blood.V97.2.426