Human cytomegalovirus and human immunodeficiency virus type-1 co-infection in human cervical tissue

Andrea M. Fox-Canale; Thomas J. Hope; Jeffrey Martinson; John R. Lurain; Alfred W. Rademaker; James W. Bremer; Alan Landay; Gregory T. Spear; Nell S. Lurain

doi:10.1016/j.virol.2007.07.008

Human cytomegalovirus and human immunodeficiency virus type-1 co-infection in human cervical tissue

Andrea M. Fox-Canale, Thomas J. Hope, Jeffrey Martinson, John R. Lurain, Alfred W. Rademaker, James W. Bremer, Alan Landay, Gregory T. Spear, Nell S. Lurain^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Human cytomegalovirus (HCMV) and human immunodeficiency virus type-1 (HIV-1) infect the female genital tract. A human cervical explant model was developed to study single and dual infection by these viruses in the genital compartment. An HCMV strain expressing green fluorescent protein, and two clinical HCMV strains produced peak viral DNA copies at 14 to 21 days post-infection. Peak levels of HIV-1_Ba-L p24 antigen occurred at 7 days post-infection. HIV-1_Ba-L appeared to enhance HCMV in co-infected tissues. Singly and dually infected explants produced increased levels of cytokines IL-6, IL-8, and GRO-α in culture supernatants. Immunohistochemical and flow cytometric analysis showed HCMV infection of leukocytes with the phenotype CD45+/CD1a+/CD14+/HLA-DR+ but not stromal or endothelial cells. Cells expressing both GFP and HIV-1 p24 antigen were detected in co-infected tissues. The cervical explants provide an ex vivo human model for examining mechanisms of virus-virus interaction and pathogenesis in clinically relevant tissue.

Original language	English (US)
Pages (from-to)	55-68
Number of pages	14
Journal	Virology
Volume	369
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2007.07.008
State	Published - Dec 5 2007

Funding

This work was supported in part by HD40539 to A.L. and T.J.H., N01-A1-50044 to J.W.B., and AI48073 subcontract to N.S.L. The authors thank Carl Ware for helpful discussions.

Keywords

Cervical explant
Female genital tract
HIV-1
Human cytomegalovirus

ASJC Scopus subject areas

Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virol.2007.07.008

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title = "Human cytomegalovirus and human immunodeficiency virus type-1 co-infection in human cervical tissue",

abstract = "Human cytomegalovirus (HCMV) and human immunodeficiency virus type-1 (HIV-1) infect the female genital tract. A human cervical explant model was developed to study single and dual infection by these viruses in the genital compartment. An HCMV strain expressing green fluorescent protein, and two clinical HCMV strains produced peak viral DNA copies at 14 to 21 days post-infection. Peak levels of HIV-1Ba-L p24 antigen occurred at 7 days post-infection. HIV-1Ba-L appeared to enhance HCMV in co-infected tissues. Singly and dually infected explants produced increased levels of cytokines IL-6, IL-8, and GRO-α in culture supernatants. Immunohistochemical and flow cytometric analysis showed HCMV infection of leukocytes with the phenotype CD45+/CD1a+/CD14+/HLA-DR+ but not stromal or endothelial cells. Cells expressing both GFP and HIV-1 p24 antigen were detected in co-infected tissues. The cervical explants provide an ex vivo human model for examining mechanisms of virus-virus interaction and pathogenesis in clinically relevant tissue.",

keywords = "Cervical explant, Female genital tract, HIV-1, Human cytomegalovirus",

author = "Fox-Canale, {Andrea M.} and Hope, {Thomas J.} and Jeffrey Martinson and Lurain, {John R.} and Rademaker, {Alfred W.} and Bremer, {James W.} and Alan Landay and Spear, {Gregory T.} and Lurain, {Nell S.}",

note = "Funding Information: This work was supported in part by HD40539 to A.L. and T.J.H., N01-A1-50044 to J.W.B., and AI48073 subcontract to N.S.L. The authors thank Carl Ware for helpful discussions.",

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T1 - Human cytomegalovirus and human immunodeficiency virus type-1 co-infection in human cervical tissue

AU - Fox-Canale, Andrea M.

AU - Hope, Thomas J.

AU - Martinson, Jeffrey

AU - Lurain, John R.

AU - Rademaker, Alfred W.

AU - Bremer, James W.

AU - Landay, Alan

AU - Spear, Gregory T.

AU - Lurain, Nell S.

N1 - Funding Information: This work was supported in part by HD40539 to A.L. and T.J.H., N01-A1-50044 to J.W.B., and AI48073 subcontract to N.S.L. The authors thank Carl Ware for helpful discussions.

PY - 2007/12/5

Y1 - 2007/12/5

N2 - Human cytomegalovirus (HCMV) and human immunodeficiency virus type-1 (HIV-1) infect the female genital tract. A human cervical explant model was developed to study single and dual infection by these viruses in the genital compartment. An HCMV strain expressing green fluorescent protein, and two clinical HCMV strains produced peak viral DNA copies at 14 to 21 days post-infection. Peak levels of HIV-1Ba-L p24 antigen occurred at 7 days post-infection. HIV-1Ba-L appeared to enhance HCMV in co-infected tissues. Singly and dually infected explants produced increased levels of cytokines IL-6, IL-8, and GRO-α in culture supernatants. Immunohistochemical and flow cytometric analysis showed HCMV infection of leukocytes with the phenotype CD45+/CD1a+/CD14+/HLA-DR+ but not stromal or endothelial cells. Cells expressing both GFP and HIV-1 p24 antigen were detected in co-infected tissues. The cervical explants provide an ex vivo human model for examining mechanisms of virus-virus interaction and pathogenesis in clinically relevant tissue.

AB - Human cytomegalovirus (HCMV) and human immunodeficiency virus type-1 (HIV-1) infect the female genital tract. A human cervical explant model was developed to study single and dual infection by these viruses in the genital compartment. An HCMV strain expressing green fluorescent protein, and two clinical HCMV strains produced peak viral DNA copies at 14 to 21 days post-infection. Peak levels of HIV-1Ba-L p24 antigen occurred at 7 days post-infection. HIV-1Ba-L appeared to enhance HCMV in co-infected tissues. Singly and dually infected explants produced increased levels of cytokines IL-6, IL-8, and GRO-α in culture supernatants. Immunohistochemical and flow cytometric analysis showed HCMV infection of leukocytes with the phenotype CD45+/CD1a+/CD14+/HLA-DR+ but not stromal or endothelial cells. Cells expressing both GFP and HIV-1 p24 antigen were detected in co-infected tissues. The cervical explants provide an ex vivo human model for examining mechanisms of virus-virus interaction and pathogenesis in clinically relevant tissue.

KW - Cervical explant

KW - Female genital tract

KW - HIV-1

KW - Human cytomegalovirus

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Human cytomegalovirus and human immunodeficiency virus type-1 co-infection in human cervical tissue

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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