Human endomucin is an endothelial marker

Canhui Liu, Zhi Ming Shao, Liping Zhang, Perrin Beatty, Maryam Sartippour, Timothy F Lane, Edward Livingston, Mai Nguyen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

The identification of novel endothelial markers is important in the study of angiogenesis, and may have potential uses in cancer diagnosis and treatment. We have isolated potential markers of tumor angiogenesis by screening human umbilical vein endothelial cells (HUVECs) treated with tumor conditioned media. Using suppression subtractive hybridization (SSH), we found endomucin, a potential cell surface marker upregulated in this system. Human endomucin is predicted to encode a 261-aa, 27.5-kDa protein with a transmembrane sequence and multiple glycosylation sites. Northern and in situ hybridization studies show that human endomucin expression is largely, if not uniquely, endothelial cell-specific. Human endomucin is present abundantly in highly vascular tissues such as heart, kidney, and lung. It is seen in human aortic endothelial cells (HAECs) as well as in human microvascular endothelial cells (HMVECs). Furthermore, its expression is increased when endothelial cells are proliferating or are stimulated by tumor-conditioned media or specific angiogenic factors such as bFGF (basic fibroblast growth factor) and TNFα (tumor necrosis factor), suggesting that endomucin may have a role in tumor angiogenesis.

Original languageEnglish (US)
Pages (from-to)129-136
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume288
Issue number1
DOIs
StatePublished - Oct 19 2001

Keywords

  • Breast
  • Endomucin
  • Endothelium
  • Tumor angiogenesis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Human endomucin is an endothelial marker'. Together they form a unique fingerprint.

Cite this