Human extrahepatic portal vein obstruction correlates with decreased factor VII and protein C transcription but increased hepatocyte proliferation

Bill Chiu, Hector Melin-Aldana, Riccardo A Superina*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Purpose: A 3-year-old girl developed extrahepatic portal vein obstruction (EHPVO) after a liver transplant. She had sequelae of portal hypertension that required another transplantation. The circumstances allowed for comparison of liver-dependent coagulation factor production between the second donor liver and the explanted liver with EHPVO. Methods: Liver samples from the explanted first graft and the second transplant were obtained. Fresh tissue was used to perform reverse transcription-polymerase chain reaction with primers against factors V, VII, as well as VIII, protein C, and paraffin-embedded sections for hepatocyte proliferation using Ki-67 antibody as well as for apoptosis using TUNEL assay. Results: The transcription of factor VII and that of protein C were decreased in the explant as compared with the newly transplanted liver (factor VII, 77% of the donor; protein C, 88% of the donor). The transcription of factor V and that of factor VIII were unchanged. The explant had a greater percentage of proliferating hepatocytes than the new organ (0.85% ± 0.75% vs 0.11% ± 0.21%). The percentage of apoptotic cells was similar between the 2 livers (0.09% ± 0.13% vs 0.09% ± 0.13%). Conclusions: Idiopathic EHPVO is associated with a reduction in liver-dependent coagulation factor transcription and an increase in hepatocyte proliferation. Portal blood flow deprivation alters hepatic homeostasis and initiates mechanisms that attempt to restore liver-dependent coagulation factors.

Original languageEnglish (US)
Pages (from-to)1768-1771
Number of pages4
JournalJournal of pediatric surgery
Volume42
Issue number10
DOIs
StatePublished - Oct 1 2007

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Keywords

  • Apoptosis
  • Coagulation factor
  • Extrahepatic portal vein obstruction
  • Proliferation

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health

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