Human extrahepatic portal vein obstruction correlates with decreased factor VII and protein C transcription but increased hepatocyte proliferation

Bill Chiu, Hector Melin-Aldana, Riccardo A Superina*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: A 3-year-old girl developed extrahepatic portal vein obstruction (EHPVO) after a liver transplant. She had sequelae of portal hypertension that required another transplantation. The circumstances allowed for comparison of liver-dependent coagulation factor production between the second donor liver and the explanted liver with EHPVO. Methods: Liver samples from the explanted first graft and the second transplant were obtained. Fresh tissue was used to perform reverse transcription-polymerase chain reaction with primers against factors V, VII, as well as VIII, protein C, and paraffin-embedded sections for hepatocyte proliferation using Ki-67 antibody as well as for apoptosis using TUNEL assay. Results: The transcription of factor VII and that of protein C were decreased in the explant as compared with the newly transplanted liver (factor VII, 77% of the donor; protein C, 88% of the donor). The transcription of factor V and that of factor VIII were unchanged. The explant had a greater percentage of proliferating hepatocytes than the new organ (0.85% ± 0.75% vs 0.11% ± 0.21%). The percentage of apoptotic cells was similar between the 2 livers (0.09% ± 0.13% vs 0.09% ± 0.13%). Conclusions: Idiopathic EHPVO is associated with a reduction in liver-dependent coagulation factor transcription and an increase in hepatocyte proliferation. Portal blood flow deprivation alters hepatic homeostasis and initiates mechanisms that attempt to restore liver-dependent coagulation factors.

Original languageEnglish (US)
Pages (from-to)1768-1771
Number of pages4
JournalJournal of Pediatric Surgery
Volume42
Issue number10
DOIs
StatePublished - Oct 1 2007

Fingerprint

Factor VII
Portal Vein
Protein C
Hepatocytes
Liver
Blood Coagulation Factors
Factor V
Factor VIII
Tissue Donors
Transplants
Transcription Factors
In Situ Nick-End Labeling
Portal Hypertension
Paraffin
Reverse Transcription
Homeostasis
Transplantation
Apoptosis
Polymerase Chain Reaction

Keywords

  • Apoptosis
  • Coagulation factor
  • Extrahepatic portal vein obstruction
  • Proliferation

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Surgery

Cite this

@article{775048dd21b642ae9e659bce40d42aa9,
title = "Human extrahepatic portal vein obstruction correlates with decreased factor VII and protein C transcription but increased hepatocyte proliferation",
abstract = "Purpose: A 3-year-old girl developed extrahepatic portal vein obstruction (EHPVO) after a liver transplant. She had sequelae of portal hypertension that required another transplantation. The circumstances allowed for comparison of liver-dependent coagulation factor production between the second donor liver and the explanted liver with EHPVO. Methods: Liver samples from the explanted first graft and the second transplant were obtained. Fresh tissue was used to perform reverse transcription-polymerase chain reaction with primers against factors V, VII, as well as VIII, protein C, and paraffin-embedded sections for hepatocyte proliferation using Ki-67 antibody as well as for apoptosis using TUNEL assay. Results: The transcription of factor VII and that of protein C were decreased in the explant as compared with the newly transplanted liver (factor VII, 77{\%} of the donor; protein C, 88{\%} of the donor). The transcription of factor V and that of factor VIII were unchanged. The explant had a greater percentage of proliferating hepatocytes than the new organ (0.85{\%} ± 0.75{\%} vs 0.11{\%} ± 0.21{\%}). The percentage of apoptotic cells was similar between the 2 livers (0.09{\%} ± 0.13{\%} vs 0.09{\%} ± 0.13{\%}). Conclusions: Idiopathic EHPVO is associated with a reduction in liver-dependent coagulation factor transcription and an increase in hepatocyte proliferation. Portal blood flow deprivation alters hepatic homeostasis and initiates mechanisms that attempt to restore liver-dependent coagulation factors.",
keywords = "Apoptosis, Coagulation factor, Extrahepatic portal vein obstruction, Proliferation",
author = "Bill Chiu and Hector Melin-Aldana and Superina, {Riccardo A}",
year = "2007",
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doi = "10.1016/j.jpedsurg.2007.06.016",
language = "English (US)",
volume = "42",
pages = "1768--1771",
journal = "Journal of Pediatric Surgery",
issn = "0022-3468",
publisher = "W.B. Saunders Ltd",
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TY - JOUR

T1 - Human extrahepatic portal vein obstruction correlates with decreased factor VII and protein C transcription but increased hepatocyte proliferation

AU - Chiu, Bill

AU - Melin-Aldana, Hector

AU - Superina, Riccardo A

PY - 2007/10/1

Y1 - 2007/10/1

N2 - Purpose: A 3-year-old girl developed extrahepatic portal vein obstruction (EHPVO) after a liver transplant. She had sequelae of portal hypertension that required another transplantation. The circumstances allowed for comparison of liver-dependent coagulation factor production between the second donor liver and the explanted liver with EHPVO. Methods: Liver samples from the explanted first graft and the second transplant were obtained. Fresh tissue was used to perform reverse transcription-polymerase chain reaction with primers against factors V, VII, as well as VIII, protein C, and paraffin-embedded sections for hepatocyte proliferation using Ki-67 antibody as well as for apoptosis using TUNEL assay. Results: The transcription of factor VII and that of protein C were decreased in the explant as compared with the newly transplanted liver (factor VII, 77% of the donor; protein C, 88% of the donor). The transcription of factor V and that of factor VIII were unchanged. The explant had a greater percentage of proliferating hepatocytes than the new organ (0.85% ± 0.75% vs 0.11% ± 0.21%). The percentage of apoptotic cells was similar between the 2 livers (0.09% ± 0.13% vs 0.09% ± 0.13%). Conclusions: Idiopathic EHPVO is associated with a reduction in liver-dependent coagulation factor transcription and an increase in hepatocyte proliferation. Portal blood flow deprivation alters hepatic homeostasis and initiates mechanisms that attempt to restore liver-dependent coagulation factors.

AB - Purpose: A 3-year-old girl developed extrahepatic portal vein obstruction (EHPVO) after a liver transplant. She had sequelae of portal hypertension that required another transplantation. The circumstances allowed for comparison of liver-dependent coagulation factor production between the second donor liver and the explanted liver with EHPVO. Methods: Liver samples from the explanted first graft and the second transplant were obtained. Fresh tissue was used to perform reverse transcription-polymerase chain reaction with primers against factors V, VII, as well as VIII, protein C, and paraffin-embedded sections for hepatocyte proliferation using Ki-67 antibody as well as for apoptosis using TUNEL assay. Results: The transcription of factor VII and that of protein C were decreased in the explant as compared with the newly transplanted liver (factor VII, 77% of the donor; protein C, 88% of the donor). The transcription of factor V and that of factor VIII were unchanged. The explant had a greater percentage of proliferating hepatocytes than the new organ (0.85% ± 0.75% vs 0.11% ± 0.21%). The percentage of apoptotic cells was similar between the 2 livers (0.09% ± 0.13% vs 0.09% ± 0.13%). Conclusions: Idiopathic EHPVO is associated with a reduction in liver-dependent coagulation factor transcription and an increase in hepatocyte proliferation. Portal blood flow deprivation alters hepatic homeostasis and initiates mechanisms that attempt to restore liver-dependent coagulation factors.

KW - Apoptosis

KW - Coagulation factor

KW - Extrahepatic portal vein obstruction

KW - Proliferation

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