Abstract
Purpose: A 3-year-old girl developed extrahepatic portal vein obstruction (EHPVO) after a liver transplant. She had sequelae of portal hypertension that required another transplantation. The circumstances allowed for comparison of liver-dependent coagulation factor production between the second donor liver and the explanted liver with EHPVO. Methods: Liver samples from the explanted first graft and the second transplant were obtained. Fresh tissue was used to perform reverse transcription-polymerase chain reaction with primers against factors V, VII, as well as VIII, protein C, and paraffin-embedded sections for hepatocyte proliferation using Ki-67 antibody as well as for apoptosis using TUNEL assay. Results: The transcription of factor VII and that of protein C were decreased in the explant as compared with the newly transplanted liver (factor VII, 77% of the donor; protein C, 88% of the donor). The transcription of factor V and that of factor VIII were unchanged. The explant had a greater percentage of proliferating hepatocytes than the new organ (0.85% ± 0.75% vs 0.11% ± 0.21%). The percentage of apoptotic cells was similar between the 2 livers (0.09% ± 0.13% vs 0.09% ± 0.13%). Conclusions: Idiopathic EHPVO is associated with a reduction in liver-dependent coagulation factor transcription and an increase in hepatocyte proliferation. Portal blood flow deprivation alters hepatic homeostasis and initiates mechanisms that attempt to restore liver-dependent coagulation factors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1768-1771 |
| Number of pages | 4 |
| Journal | Journal of Pediatric Surgery |
| Volume | 42 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 1 2007 |
Fingerprint
Keywords
- Apoptosis
- Coagulation factor
- Extrahepatic portal vein obstruction
- Proliferation
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
- Surgery
Cite this
}
Human extrahepatic portal vein obstruction correlates with decreased factor VII and protein C transcription but increased hepatocyte proliferation. / Chiu, Bill; Melin-Aldana, Hector; Superina, Riccardo A.
In: Journal of Pediatric Surgery, Vol. 42, No. 10, 01.10.2007, p. 1768-1771.Research output: Contribution to journal › Article
TY - JOUR
T1 - Human extrahepatic portal vein obstruction correlates with decreased factor VII and protein C transcription but increased hepatocyte proliferation
AU - Chiu, Bill
AU - Melin-Aldana, Hector
AU - Superina, Riccardo A
PY - 2007/10/1
Y1 - 2007/10/1
N2 - Purpose: A 3-year-old girl developed extrahepatic portal vein obstruction (EHPVO) after a liver transplant. She had sequelae of portal hypertension that required another transplantation. The circumstances allowed for comparison of liver-dependent coagulation factor production between the second donor liver and the explanted liver with EHPVO. Methods: Liver samples from the explanted first graft and the second transplant were obtained. Fresh tissue was used to perform reverse transcription-polymerase chain reaction with primers against factors V, VII, as well as VIII, protein C, and paraffin-embedded sections for hepatocyte proliferation using Ki-67 antibody as well as for apoptosis using TUNEL assay. Results: The transcription of factor VII and that of protein C were decreased in the explant as compared with the newly transplanted liver (factor VII, 77% of the donor; protein C, 88% of the donor). The transcription of factor V and that of factor VIII were unchanged. The explant had a greater percentage of proliferating hepatocytes than the new organ (0.85% ± 0.75% vs 0.11% ± 0.21%). The percentage of apoptotic cells was similar between the 2 livers (0.09% ± 0.13% vs 0.09% ± 0.13%). Conclusions: Idiopathic EHPVO is associated with a reduction in liver-dependent coagulation factor transcription and an increase in hepatocyte proliferation. Portal blood flow deprivation alters hepatic homeostasis and initiates mechanisms that attempt to restore liver-dependent coagulation factors.
AB - Purpose: A 3-year-old girl developed extrahepatic portal vein obstruction (EHPVO) after a liver transplant. She had sequelae of portal hypertension that required another transplantation. The circumstances allowed for comparison of liver-dependent coagulation factor production between the second donor liver and the explanted liver with EHPVO. Methods: Liver samples from the explanted first graft and the second transplant were obtained. Fresh tissue was used to perform reverse transcription-polymerase chain reaction with primers against factors V, VII, as well as VIII, protein C, and paraffin-embedded sections for hepatocyte proliferation using Ki-67 antibody as well as for apoptosis using TUNEL assay. Results: The transcription of factor VII and that of protein C were decreased in the explant as compared with the newly transplanted liver (factor VII, 77% of the donor; protein C, 88% of the donor). The transcription of factor V and that of factor VIII were unchanged. The explant had a greater percentage of proliferating hepatocytes than the new organ (0.85% ± 0.75% vs 0.11% ± 0.21%). The percentage of apoptotic cells was similar between the 2 livers (0.09% ± 0.13% vs 0.09% ± 0.13%). Conclusions: Idiopathic EHPVO is associated with a reduction in liver-dependent coagulation factor transcription and an increase in hepatocyte proliferation. Portal blood flow deprivation alters hepatic homeostasis and initiates mechanisms that attempt to restore liver-dependent coagulation factors.
KW - Apoptosis
KW - Coagulation factor
KW - Extrahepatic portal vein obstruction
KW - Proliferation
UR - http://www.scopus.com/inward/record.url?scp=34848847444&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34848847444&partnerID=8YFLogxK
U2 - 10.1016/j.jpedsurg.2007.06.016
DO - 10.1016/j.jpedsurg.2007.06.016
M3 - Article
VL - 42
SP - 1768
EP - 1771
JO - Journal of Pediatric Surgery
JF - Journal of Pediatric Surgery
SN - 0022-3468
IS - 10
ER -