Human Fibroblasts (KMST-6/Ras Cell Line) Transformed with 60Co Gamma-Rays and C-Ha-ras Oncogene Produce a Large Amount of Granulocyte Colony-Stimulating Factor(G-CSF); Production Is Enhanced by cAMP, Theophylline, and Butyrate

Kunihiro Kawashima, Israt Jahan, Kazuo Fushimi, Koichiro Mihara, Masayoshi Namba*

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Human fibroblasts (KMST-6/RAS cell line), which was malignantly transformed in vitro with 60Co gamma-rays and the c-Ha-ras oncogene, produced a large amount of granulocyte colony-stimulating factor (G-CSF). The production was greater during the logarithmic growth phase than during the stationary phase. cAMP and theophylline, alone or in combination, and butyrate significantly enhanced G-CSF production, but dexamethasone or 5-azacytidine did not. Enhanced production of G-CSF by these agents was regulated at the posttranscriptional level. Neither the expression of the ras oncogene nor the tumorigenicity of the cells correlated with the production of G-CSF.

Original languageEnglish (US)
Pages (from-to)41-45
Number of pages5
JournalCell Structure and Function
Volume20
Issue number1
DOIs
StatePublished - Jan 1 1995

Keywords

  • G-CSF
  • butyrate
  • cAMP
  • human fibroblasts

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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