Human kallikrein 7 induces epithelial-mesenchymal transition-like changes in prostate carcinoma cells: A role in prostate cancer invasion and progression

Linjian Mo, Ju Zhang, Jiandang Shi, Qiang Xuan, Xiaoli Yang, Min Qin, Chung Lee, Helmut Klocker, Qingdi Quentin Li, Zengnan Mo

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Human tissue kallikrein 7 (hK7), a chymotrypsin-like secreted serine protease, catalyzes the degradation of intercellular adhesive structures in the cornified layer of the skin, leading to desquamation. Thus, hK7 is implicated in cancer invasion and metastasis. Although hK7 is highly expressed in prostate tissues, its biological role in prostate cancer progression is poorly understood. In the current study, we established an hK7-expressing cell model for prostate tumors by stably transfecting prostate carcinoma 22RV1 and DU145 cells with an expression vector encoding hK7. We found that there were no obvious differences in cell proliferation between cells overexpressing hK7 and cells transfected with empty vector (p>0.05). Intriguingly, a Matrigel invasion assay revealed that hK7 remarkably increased the migration and invasion of prostate cancer cells (p<0.01). Furthermore, hK7 induced epithelial-mesenchymal transition-like changes in prostate carcinoma cells, as evidenced by scattered cellular growth, mesenchyma-like morphology, and up-regulated expression of vimentin, a mesenchymal marker. These novel findings suggest that hK7 plays an important role in mediating prostate cancer progression and that hK7 promotes invasion and metastasis, at least in part, through inducing the epithelial-mesenchymal transition of prostatic carcinoma cells.

Original languageEnglish (US)
Pages (from-to)3413-3420
Number of pages8
JournalAnticancer research
Volume30
Issue number9
StatePublished - Jan 1 2010

Keywords

  • Human tissue kallikrein 7
  • Invasion
  • Metastasis
  • Morphological change
  • Prostate cancer
  • Vimentin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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