Human menopausal gonadotropin during in vitro maturation of human oocytes retrieved from small follicles enhances in vitro fertilization and cleavage rates

X. Zhang*, A. Zerafa, J. Wong, D. T. Armstrong, F. Khamsi

*Corresponding author for this work

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Objectives: To compare the IVF rates of oocytes retrieved from small follicles (<2 mL in volume) with those of oocytes retrieved from large follicles and to test the effect of adding gonadotropins to the IVF medium on the fertilization rates of oocytes from small follicles. Design: Oocytes were retrieved with endovaginal ultrasound (US) guidance from patients undergoing infertility treatment in our IVF program. Oocytes were grouped according to the volume of the originating follicle and subjected to our routine procedure for IVF. HMG was added to the IVF medium for some of the oocytes from small follicles. Setting: Toronto Fertility and Sterility Institute is affiliated with the University of Western Ontario and University of Toronto and is equipped for RIA, endovaginal US monitoring and oocyte retrieval, and for processing and culturing gametes and embryos. Patients: Infertile patients admitted to our IVF program. Interventions: Patients underwent ovarian stimulation with hMG before oocytes retrieval. No other interventions were introduced to the processing and culturing the gametes and embryos except the addition of hMG to the medium of some of the small follicle-originated oocytes with the informed consent from the patients. Main Outcome Measures: Rates of fertilization, cleavage of the fertilized embryos before replacement, and meiotic status of some of the oocytes from small follicles. Results: Most of the oocytes from small follicles did not complete the first meiotic division; they had low rates of fertilization and cleavage compared with oocytes from large follicles, and these rates were improved by the addition of hMG to the IVF medium. Conclusions: Oocytes from small follicles are probably less mature and require a more physiological environment to achieve normal rates of fertilization and cleavage.

Original languageEnglish (US)
Pages (from-to)850-853
Number of pages4
JournalFertility and Sterility
Volume59
Issue number4
DOIs
StatePublished - Jan 1 1993

Keywords

  • Oocyte maturation
  • follicular volume
  • gonadotropins
  • in vitro fertilization

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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