Human mitochondrial electron transport chain: Assay of succinate:Cytochrome c reductase in leukocytes, platelets and cultured fibroblasts

David A. Stumpf*, Janice K. Parks

*Corresponding author for this work

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

An assay for the middle portion of the mitochondrial electron transport chain (ETC) is described. Succinate: cytochrome c reductase (SCR) activity is measured in mitochondria isolated from human leukocytes, platelets, and cultured fibroblasts. The activity is blocked by antimycin A indicating specificity for the mitochondrial inner membrane ETC. SCR values in normal and Friedreich's ataxia patients were similar. This assay may be helpful in evaluating acquired and heritable disorders of mitochondrial metabolism.

Original languageEnglish (US)
Pages (from-to)234-238
Number of pages5
JournalBiochemical medicine
Volume25
Issue number2
DOIs
StatePublished - Apr 1981

ASJC Scopus subject areas

  • Biochemistry

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