Human severe combined immunodeficiency due to a defect in ZAP-70, a T cell tyrosine kinase

A homozygous mutation in the kinase domain of ZAP-70, a T cell receptor-associated protein tyrosine kinase, produced a distinctive form of human severe combined immunodeficiency. Manifestations of this disorder included profound immunodeficiency, absence of peripheral CD8⁺ T cells, and abundant peripheral CD4⁺ T cells that were refractory to T cell receptor-mediated activation. These findings demonstrate that ZAP-70 is essential for human T cell function and suggest that CD4⁺ and CDS⁺ T cells depend on different intracellular signaling pathways to support their development or survival.