Human Sperm Aneuploidy after Exposure to Pesticides

Jena L. Smith, Vincent F. Garry, Alfred W. Rademaker, Renée H. Martin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


This study examined the effect of paternal environmental exposure to pesticides on the frequency of aneuploidy in human sperm. To determine if the chromosome number in germ cells was altered by paternal exposure, multicolor fluorescence in situ hybridization (FISH) analysis was utilized to measure aneuploidy frequencies in the sperm of 40 men (20 exposed, 20 controls). Samples were coded for "blind analysis" to eliminate scorer bias. Aneuploidy and diploidy frequencies were assessed for chromosomes 13, 21, X, and Y. A minimum of 10,000 sperm was scored per donor per chromosome probe with a total of 809,935 sperm scored. Hybridization efficiency was 99%. There were no significant differences in aneuploidy or diploidy frequencies between exposed and control groups, suggesting that the pesticides did not increase the risk of numerical chromosomal abnormalities in these men.

Original languageEnglish (US)
Pages (from-to)353-359
Number of pages7
JournalMolecular reproduction and development
Issue number3
StatePublished - Mar 2004


  • Aneuploidy
  • Chromosomal abnormalities
  • Fluorescence in situ hybridization
  • Pesticide exposure

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology


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