Human testis cdnas identified by sera from infertile patients: A molecular biological approach to immunocontraceptive development

Z. G. Liang; P. A. O’Hem; B. Yavetz; H. Yavetz; E. Goldberg

doi:10.1071/RD9940297

Human testis cdnas identified by sera from infertile patients: A molecular biological approach to immunocontraceptive development

Z. G. Liang, P. A. O’Hem, B. Yavetz, H. Yavetz, E. Goldberg

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Sera from patients with known or suspected immunological infertility were used to screen a human testis cDNA library. A total of 59 sera detected 38 unique cDNA inserts of which four were testis specific by Northern blot analyses. One of these is a testis-specific isoform of calpastatin. Five additional clones, although not testis specific, were found to be testis abundant. The number and type of clones identified by these human sera suggests a possible aetiology for immunologic infertility. The testis-specific clones will be further characterized to establish their usefulness as contraceptive vaccine candidates.

Original language	English (US)
Pages (from-to)	297-305
Number of pages	9
Journal	Reproduction, Fertility and Development
Volume	6
Issue number	3
DOIs	https://doi.org/10.1071/RD9940297
State	Published - 1994

ASJC Scopus subject areas

Biotechnology
Reproductive Medicine
Animal Science and Zoology
Molecular Biology
Genetics
Endocrinology
Developmental Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1071/RD9940297

Cite this

@article{c357656ee6c2444bb8eebb3ddb1a21d2,

title = "Human testis cdnas identified by sera from infertile patients: A molecular biological approach to immunocontraceptive development",

abstract = "Sera from patients with known or suspected immunological infertility were used to screen a human testis cDNA library. A total of 59 sera detected 38 unique cDNA inserts of which four were testis specific by Northern blot analyses. One of these is a testis-specific isoform of calpastatin. Five additional clones, although not testis specific, were found to be testis abundant. The number and type of clones identified by these human sera suggests a possible aetiology for immunologic infertility. The testis-specific clones will be further characterized to establish their usefulness as contraceptive vaccine candidates.",

author = "Liang, {Z. G.} and O{\textquoteright}Hem, {P. A.} and B. Yavetz and H. Yavetz and E. Goldberg",

note = "Funding Information: This work was supported in part by a subcontract under grant U54 HD 29099 from the National Institutes of Health. H. Y. was an Andrew W. Mellon Foundation Fellow. We thank T. Hjort, R. Bronson and J. Overstreet for providing the sera used in these studies, Alan Diekrnan and Christophe Bonny for advice, and Martine Benoit for editorial assistance in preparing the manuscript.",

year = "1994",

doi = "10.1071/RD9940297",

language = "English (US)",

volume = "6",

pages = "297--305",

journal = "Reproduction, Fertility and Development",

issn = "1031-3613",

publisher = "CSIRO",

number = "3",

}

TY - JOUR

T1 - Human testis cdnas identified by sera from infertile patients

T2 - A molecular biological approach to immunocontraceptive development

AU - Liang, Z. G.

AU - O’Hem, P. A.

AU - Yavetz, B.

AU - Yavetz, H.

AU - Goldberg, E.

N1 - Funding Information: This work was supported in part by a subcontract under grant U54 HD 29099 from the National Institutes of Health. H. Y. was an Andrew W. Mellon Foundation Fellow. We thank T. Hjort, R. Bronson and J. Overstreet for providing the sera used in these studies, Alan Diekrnan and Christophe Bonny for advice, and Martine Benoit for editorial assistance in preparing the manuscript.

PY - 1994

Y1 - 1994

N2 - Sera from patients with known or suspected immunological infertility were used to screen a human testis cDNA library. A total of 59 sera detected 38 unique cDNA inserts of which four were testis specific by Northern blot analyses. One of these is a testis-specific isoform of calpastatin. Five additional clones, although not testis specific, were found to be testis abundant. The number and type of clones identified by these human sera suggests a possible aetiology for immunologic infertility. The testis-specific clones will be further characterized to establish their usefulness as contraceptive vaccine candidates.

AB - Sera from patients with known or suspected immunological infertility were used to screen a human testis cDNA library. A total of 59 sera detected 38 unique cDNA inserts of which four were testis specific by Northern blot analyses. One of these is a testis-specific isoform of calpastatin. Five additional clones, although not testis specific, were found to be testis abundant. The number and type of clones identified by these human sera suggests a possible aetiology for immunologic infertility. The testis-specific clones will be further characterized to establish their usefulness as contraceptive vaccine candidates.

UR - http://www.scopus.com/inward/record.url?scp=0028145399&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028145399&partnerID=8YFLogxK

U2 - 10.1071/RD9940297

DO - 10.1071/RD9940297

M3 - Article

C2 - 7530381

AN - SCOPUS:0028145399

SN - 1031-3613

VL - 6

SP - 297

EP - 305

JO - Reproduction, Fertility and Development

JF - Reproduction, Fertility and Development

IS - 3

ER -

Human testis cdnas identified by sera from infertile patients: A molecular biological approach to immunocontraceptive development

Abstract

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this