Human umbilical cord blood cells can be induced to express markers for neurons and glia

A. R. Bicknese*, H. S. Goodwin, C. O. Quinn, V. C D Henderson, S. N. Chien, D. A. Wall

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

96 Scopus citations

Abstract

Rare cells are present in human umbilical cord blood that do not express the hematopoietic marker CD45 and in culture do not produce cells of hematopoietic lineage. These umbilical cord multipotent stem cells (UC-MC) behave as multilineage progenitor cells (stem cells) and can be expanded in tissue culture. Exposure to basic fibroblast growth factor (bFGF) and human epidermal growth factor (hEGF) for a minimum of 7 days in culture induces expression of neural and glial markers. Western immunoblots demonstrate expression of both β-tubulin III and glial fibrillary acidic protein (GFAP). Immunocytochemistry of the cells showed intense labeling to both compounds on the intracellular cytoskeleton. The oligodendrocyte cell surface marker galactocerebroside (Gal-C) was present on most cells. Many cells show dual labeling, expressing both neuronal and glial markers.

Original languageEnglish (US)
Pages (from-to)261-264
Number of pages4
JournalCell transplantation
Volume11
Issue number3
DOIs
StatePublished - 2002

Keywords

  • Astrocyte
  • Glia
  • Lineage
  • Neuron
  • Stem cell
  • Umbilical cord blood

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

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