Human urate oxidase gene: Cloning and partial sequence analysis reveal a stop codon within the fifth exon

Anjana V. Yeldandi; Xuedong Wang; Keith Alvares; Sujata Kumar; M. Sambasiva Rao; Janardan K. Reddy

doi:10.1016/0006-291X(90)91194-W

Human urate oxidase gene: Cloning and partial sequence analysis reveal a stop codon within the fifth exon

Anjana V. Yeldandi^*, Xuedong Wang, Keith Alvares, Sujata Kumar, M. Sambasiva Rao, Janardan K. Reddy

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Using the cDNA and selected genomic probes of rat urate oxidase, we have screened the human genomic library and isolated seven clones; one clone (clone 13) contained exonic regions which correspond to the exons 5, 6, and 7 of rat urate oxidase gene. The nucleotide sequence was determined for these three exons and exon/intron junctions, and compared with the sequence from the rat gene. A mutation resulting in a stop codon TGA was found in the fifth exon of the human urate oxidase gene. Sequence analysis of the polymerase chain reaction amplified DNA, corresponding to the fifth exon of urate oxidase from DNA samples from four different individuals, confirmed the same TGA stop codon in all. This single stop codon mutation and/or other mutation(s) in this gene may be responsible for the lack of urate oxidase activity in the human.

Original language	English (US)
Pages (from-to)	641-646
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	171
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(90)91194-W
State	Published - Sep 14 1990

Funding

We thank Ms. K. Stenson for excellent secretarial assistance and Mr. Mohammed I. Usman for valuable technical assistance. This research was supported by U.S. Public Health Service Grant NIH R37 GM23750 (Merii Award to JKR).

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

Access to Document

10.1016/0006-291X(90)91194-W

Cite this

@article{d42081c6981343f4ad8fbf9d93241d75,

title = "Human urate oxidase gene: Cloning and partial sequence analysis reveal a stop codon within the fifth exon",

abstract = "Using the cDNA and selected genomic probes of rat urate oxidase, we have screened the human genomic library and isolated seven clones; one clone (clone 13) contained exonic regions which correspond to the exons 5, 6, and 7 of rat urate oxidase gene. The nucleotide sequence was determined for these three exons and exon/intron junctions, and compared with the sequence from the rat gene. A mutation resulting in a stop codon TGA was found in the fifth exon of the human urate oxidase gene. Sequence analysis of the polymerase chain reaction amplified DNA, corresponding to the fifth exon of urate oxidase from DNA samples from four different individuals, confirmed the same TGA stop codon in all. This single stop codon mutation and/or other mutation(s) in this gene may be responsible for the lack of urate oxidase activity in the human.",

author = "Yeldandi, {Anjana V.} and Xuedong Wang and Keith Alvares and Sujata Kumar and Rao, {M. Sambasiva} and Reddy, {Janardan K.}",

note = "Funding Information: We thank Ms. K. Stenson for excellent secretarial assistance and Mr. Mohammed I. Usman for valuable technical assistance. This research was supported by U.S. Public Health Service Grant NIH R37 GM23750 (Merii Award to JKR).",

year = "1990",

month = sep,

day = "14",

doi = "10.1016/0006-291X(90)91194-W",

language = "English (US)",

volume = "171",

pages = "641--646",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Human urate oxidase gene

T2 - Cloning and partial sequence analysis reveal a stop codon within the fifth exon

AU - Yeldandi, Anjana V.

AU - Wang, Xuedong

AU - Alvares, Keith

AU - Kumar, Sujata

AU - Rao, M. Sambasiva

AU - Reddy, Janardan K.

N1 - Funding Information: We thank Ms. K. Stenson for excellent secretarial assistance and Mr. Mohammed I. Usman for valuable technical assistance. This research was supported by U.S. Public Health Service Grant NIH R37 GM23750 (Merii Award to JKR).

PY - 1990/9/14

Y1 - 1990/9/14

N2 - Using the cDNA and selected genomic probes of rat urate oxidase, we have screened the human genomic library and isolated seven clones; one clone (clone 13) contained exonic regions which correspond to the exons 5, 6, and 7 of rat urate oxidase gene. The nucleotide sequence was determined for these three exons and exon/intron junctions, and compared with the sequence from the rat gene. A mutation resulting in a stop codon TGA was found in the fifth exon of the human urate oxidase gene. Sequence analysis of the polymerase chain reaction amplified DNA, corresponding to the fifth exon of urate oxidase from DNA samples from four different individuals, confirmed the same TGA stop codon in all. This single stop codon mutation and/or other mutation(s) in this gene may be responsible for the lack of urate oxidase activity in the human.

AB - Using the cDNA and selected genomic probes of rat urate oxidase, we have screened the human genomic library and isolated seven clones; one clone (clone 13) contained exonic regions which correspond to the exons 5, 6, and 7 of rat urate oxidase gene. The nucleotide sequence was determined for these three exons and exon/intron junctions, and compared with the sequence from the rat gene. A mutation resulting in a stop codon TGA was found in the fifth exon of the human urate oxidase gene. Sequence analysis of the polymerase chain reaction amplified DNA, corresponding to the fifth exon of urate oxidase from DNA samples from four different individuals, confirmed the same TGA stop codon in all. This single stop codon mutation and/or other mutation(s) in this gene may be responsible for the lack of urate oxidase activity in the human.

UR - http://www.scopus.com/inward/record.url?scp=0025160235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025160235&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(90)91194-W

DO - 10.1016/0006-291X(90)91194-W

M3 - Article

C2 - 2403354

AN - SCOPUS:0025160235

SN - 0006-291X

VL - 171

SP - 641

EP - 646

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Human urate oxidase gene: Cloning and partial sequence analysis reveal a stop codon within the fifth exon

Abstract

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this