Huntingtin mediates dendritic transport of β-actin mRNA in rat neurons

Bin Ma, Jeffrey N. Savas, Man Shan Yu, Brady P. Culver, Moses V. Chao, Naoko Tanese*

*Corresponding author for this work

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Transport of mRNAs to diverse neuronal locations via RNA granules serves an important function in regulating protein synthesis within restricted sub-cellular domains. We recently detected the Huntington's disease protein huntingtin (Htt) in dendritic RNA granules; however, the functional significance of this localization is not known. Here we report that Htt and the huntingtin-associated protein 1 (HAP1) are co-localized with the microtubule motor proteins, the KIF5A kinesin and dynein, during dendritic transport of β-actin mRNA. Live cell imaging demonstrated that β-actin mRNA is associated with Htt, HAP1, and dynein intermediate chain in cultured neurons. Reduction in the levels of Htt, HAP1, KIF5A, and dynein heavy chain by lentiviral-based shRNAs resulted in a reduction in the transport of β-actin mRNA. These findings support a role for Htt in participating in the mRNA transport machinery that also contains HAP1, KIF5A, and dynein.

Original languageEnglish (US)
Article number140
JournalScientific reports
Volume1
DOIs
StatePublished - Dec 1 2011

ASJC Scopus subject areas

  • General

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