Hyaluronic acid rfx'fptors of thk human trabecular mesiiwork

Paul A Knepper*, R. Lukas, J. Wills, W. Goosscns, C. S K Mayani

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Purpose Hyaluronic acid (HA) has important regulatory functions in cellular differentiation and cell locomotion. HA receptors are diverse in structure (RHAMM. receptor hyaluronic acid mediated motility, compared with CD38, an ectoenzyme) and influence cell adhesion and reactivity (CD44, homologous to link protein: ICAM-1 [CD54]; ICAM-2 [CD102]: ICAM-3 [CD-50]; and LFA-3 [CD58]. all members of the immunoglobulin superfamily). Methods The trabecular mcshwork (TM). iris, ciliary body and sciera of normal and primary open angle glaucoma (POAG) human eyes, preserved in 100% ethanol, were microdissccted. To confirm the presence of HA receptors in the human TM, Wesiern analysis was used with antibodies against HA receptors: RHAMM (3T3-5MMAb-N). CD38, ICAM-1. ICAM-2, ICAM-3 and CD44. Jiesufcs Western blot analysis revealed: 1) ICAM-1 and ICAM-2, RHAMM and CD44 were immunopositive whereas CD38 was not detected in the normal and 2) an increase of CD44 appeared to characterize POAG particularly in the iris and ciliary body. Conclusions The human TM has several HA receptors which may influence TM cell migration as well as regenerative capacity. One HA receptor. CD44, may represent a prognostic protein marker of POAG.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - Dec 1 1997

ASJC Scopus subject areas

  • Ophthalmology

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