Hybrid membrane IgM with the transmembrane region of I-Aα facilitates enhanced presentation of distinct epitopes to T cells

Ko Jiunn Liu, Michael Schwen, Philip W. Tucker, Byung S. Kim*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The role of B cell Ag receptors (membrane Ig or mIg) in the efficient Ag presentation to T cells, including the requirement of mIgM-associated Iga/Igβ, remains unclear. We report here that mIgM, substituted with greater than two-thirds of the NH2-terminal Aα transmembrane (TM) regions of the MHC class II molecule, are capable of mediating the efficient presentation of specific Ag to some (Group 1) but not all (Group 2) T cell hybridomas. In contrast, the generation of epitopes recognized by the Group 2 hybridomas can be mediated only by the wild-type mIgM. Tyrosine phosphorylation appears to be necessary for the enhanced Ag presentation to Group 2 hybridomas, while it does not for Group 1 hybridomas. In addition, differential sensitivity of Ag processing to leupeptin, different duration required for epitope generation/presentation, as well as the involvement of distinct epitopes for stimulation of these groups of T cell hybridomas were observed. These results suggest that transport of the mIgM/Ag complexes to an endocytic compartment(s) for generation of certain T cell epitopes may be mediated by the N-terminal TM sequence of mIgM, independent of Iga/Igβ association. This function can be replaced by two-thirds of the NH2-terminal TM region of Aα chain of class H molecules.

Original languageEnglish (US)
Pages (from-to)4161-4168
Number of pages8
JournalJournal of Immunology
Volume160
Issue number9
StatePublished - May 1 1998

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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