Hybridization between the threatened plant, Lespedeza leptostachya Englem. and its co-occurring congener Lespedeza capitata Michx.: Morphological and molecular evidence

J. B. Fant; A. Banai; K. Havens; P. Vitt

doi:10.1007/s10592-010-0105-9

Hybridization between the threatened plant, Lespedeza leptostachya Englem. and its co-occurring congener Lespedeza capitata Michx. Morphological and molecular evidence

J. B. Fant, A. Banai, K. Havens, P. Vitt^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Natural hybridization is common in the genus Lespedeza. No hybrids between Lespedeza leptostachya Englem. and Lespedeza capitata Michx. are formally recognized in any of the current floras, however observations in the field suggest that hybridization might occur in many of their shared habitats. Putative hybrids were compared to L. leptostachya and L. capitata using morphological measurements and screened for the presence of species-specific trnL-F gene region (cpDNA) and the ITS gene region (nrDNA). A discriminate analysis of 10 morphological measurements identified the hybrids as intermediate to both parents with two PCA axes explaining 99% of the variation between taxa. The presence of hybrids was confirmed by genetic markers with individuals morphologically identified as hybrids having cpDNA trnL-F genotypes identical to L. leptostachya and the ITS (nrDNA) phenotypes in most cases contain the ITS genotype of both parents, however, some putative hybrid individuals contained the ITS genotype of only one parents. Those individuals with L. leptostachya ITS and trnL-F could be a case of misclassification, but the presence of both L. capitata ITS genotypes and L. leptostachyatrnL-F genotypes suggest segregation has occurred, which may result from either selfing or backcrossing.

Original language	English (US)
Pages (from-to)	2195-2205
Number of pages	11
Journal	Conservation Genetics
Volume	11
Issue number	6
DOIs	https://doi.org/10.1007/s10592-010-0105-9
State	Published - Dec 2010

Funding

Acknowledgments We wish to thank Andrea Kramer, Krissa Skogen, Ben Staehlin, Rebecca Tonietto and Nyree Zerega, on their comments on initial version of the manuscripts and two anonymous reviewers. Thanks also to Northwestern University, the Chicago Botanic Garden, the Illinois Department of Natural Resources, the Illinois Native Plant Society, and the North Shore Garden Club for funding to support this research. We thank Todd Bittner, Phil Delphy, Kristin Kordecki, Nancy Sather, Tom Sather, Eileen Sirkin, Mark Vaniman, Bill Watson, and Nyree Zerega, for field and lab assistance. Thanks also go to Dr. Paul P. Tinerella, Insect Collection Manager and Associate Research Scientist for the Illinois Natural History Survey for identifying the insects visiting our Lespedeza taxa.

Keywords

Genetic introgression
Hybrids
Lespedeza
Morphological introgression
Threatened species

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s10592-010-0105-9

Cite this

@article{ed9c218eaae24e5899cda3a13f22efca,

title = "Hybridization between the threatened plant, Lespedeza leptostachya Englem. and its co-occurring congener Lespedeza capitata Michx.: Morphological and molecular evidence",

abstract = "Natural hybridization is common in the genus Lespedeza. No hybrids between Lespedeza leptostachya Englem. and Lespedeza capitata Michx. are formally recognized in any of the current floras, however observations in the field suggest that hybridization might occur in many of their shared habitats. Putative hybrids were compared to L. leptostachya and L. capitata using morphological measurements and screened for the presence of species-specific trnL-F gene region (cpDNA) and the ITS gene region (nrDNA). A discriminate analysis of 10 morphological measurements identified the hybrids as intermediate to both parents with two PCA axes explaining 99\% of the variation between taxa. The presence of hybrids was confirmed by genetic markers with individuals morphologically identified as hybrids having cpDNA trnL-F genotypes identical to L. leptostachya and the ITS (nrDNA) phenotypes in most cases contain the ITS genotype of both parents, however, some putative hybrid individuals contained the ITS genotype of only one parents. Those individuals with L. leptostachya ITS and trnL-F could be a case of misclassification, but the presence of both L. capitata ITS genotypes and L. leptostachyatrnL-F genotypes suggest segregation has occurred, which may result from either selfing or backcrossing.",

keywords = "Genetic introgression, Hybrids, Lespedeza, Morphological introgression, Threatened species",

author = "Fant, \{J. B.\} and A. Banai and K. Havens and P. Vitt",

note = "Funding Information: Acknowledgments We wish to thank Andrea Kramer, Krissa Skogen, Ben Staehlin, Rebecca Tonietto and Nyree Zerega, on their comments on initial version of the manuscripts and two anonymous reviewers. Thanks also to Northwestern University, the Chicago Botanic Garden, the Illinois Department of Natural Resources, the Illinois Native Plant Society, and the North Shore Garden Club for funding to support this research. We thank Todd Bittner, Phil Delphy, Kristin Kordecki, Nancy Sather, Tom Sather, Eileen Sirkin, Mark Vaniman, Bill Watson, and Nyree Zerega, for field and lab assistance. Thanks also go to Dr. Paul P. Tinerella, Insect Collection Manager and Associate Research Scientist for the Illinois Natural History Survey for identifying the insects visiting our Lespedeza taxa.",

year = "2010",

month = dec,

doi = "10.1007/s10592-010-0105-9",

language = "English (US)",

volume = "11",

pages = "2195--2205",

journal = "Conservation Genetics",

issn = "1566-0621",

publisher = "Springer Science and Business Media B.V.",

number = "6",

}

TY - JOUR

T1 - Hybridization between the threatened plant, Lespedeza leptostachya Englem. and its co-occurring congener Lespedeza capitata Michx.

T2 - Morphological and molecular evidence

AU - Fant, J. B.

AU - Banai, A.

AU - Havens, K.

AU - Vitt, P.

N1 - Funding Information: Acknowledgments We wish to thank Andrea Kramer, Krissa Skogen, Ben Staehlin, Rebecca Tonietto and Nyree Zerega, on their comments on initial version of the manuscripts and two anonymous reviewers. Thanks also to Northwestern University, the Chicago Botanic Garden, the Illinois Department of Natural Resources, the Illinois Native Plant Society, and the North Shore Garden Club for funding to support this research. We thank Todd Bittner, Phil Delphy, Kristin Kordecki, Nancy Sather, Tom Sather, Eileen Sirkin, Mark Vaniman, Bill Watson, and Nyree Zerega, for field and lab assistance. Thanks also go to Dr. Paul P. Tinerella, Insect Collection Manager and Associate Research Scientist for the Illinois Natural History Survey for identifying the insects visiting our Lespedeza taxa.

PY - 2010/12

Y1 - 2010/12

N2 - Natural hybridization is common in the genus Lespedeza. No hybrids between Lespedeza leptostachya Englem. and Lespedeza capitata Michx. are formally recognized in any of the current floras, however observations in the field suggest that hybridization might occur in many of their shared habitats. Putative hybrids were compared to L. leptostachya and L. capitata using morphological measurements and screened for the presence of species-specific trnL-F gene region (cpDNA) and the ITS gene region (nrDNA). A discriminate analysis of 10 morphological measurements identified the hybrids as intermediate to both parents with two PCA axes explaining 99% of the variation between taxa. The presence of hybrids was confirmed by genetic markers with individuals morphologically identified as hybrids having cpDNA trnL-F genotypes identical to L. leptostachya and the ITS (nrDNA) phenotypes in most cases contain the ITS genotype of both parents, however, some putative hybrid individuals contained the ITS genotype of only one parents. Those individuals with L. leptostachya ITS and trnL-F could be a case of misclassification, but the presence of both L. capitata ITS genotypes and L. leptostachyatrnL-F genotypes suggest segregation has occurred, which may result from either selfing or backcrossing.

AB - Natural hybridization is common in the genus Lespedeza. No hybrids between Lespedeza leptostachya Englem. and Lespedeza capitata Michx. are formally recognized in any of the current floras, however observations in the field suggest that hybridization might occur in many of their shared habitats. Putative hybrids were compared to L. leptostachya and L. capitata using morphological measurements and screened for the presence of species-specific trnL-F gene region (cpDNA) and the ITS gene region (nrDNA). A discriminate analysis of 10 morphological measurements identified the hybrids as intermediate to both parents with two PCA axes explaining 99% of the variation between taxa. The presence of hybrids was confirmed by genetic markers with individuals morphologically identified as hybrids having cpDNA trnL-F genotypes identical to L. leptostachya and the ITS (nrDNA) phenotypes in most cases contain the ITS genotype of both parents, however, some putative hybrid individuals contained the ITS genotype of only one parents. Those individuals with L. leptostachya ITS and trnL-F could be a case of misclassification, but the presence of both L. capitata ITS genotypes and L. leptostachyatrnL-F genotypes suggest segregation has occurred, which may result from either selfing or backcrossing.

KW - Genetic introgression

KW - Hybrids

KW - Lespedeza

KW - Morphological introgression

KW - Threatened species

UR - http://www.scopus.com/inward/record.url?scp=78049399671&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78049399671&partnerID=8YFLogxK

U2 - 10.1007/s10592-010-0105-9

DO - 10.1007/s10592-010-0105-9

M3 - Article

AN - SCOPUS:78049399671

SN - 1566-0621

VL - 11

SP - 2195

EP - 2205

JO - Conservation Genetics

JF - Conservation Genetics

IS - 6

ER -