Hybridization-induced "off-on" 19F-NMR signal probe release from DNA-functionalized gold nanoparticles

Alexander Kieger; Michael J. Wiester; Daniel Procissi; Todd B. Parrish; Chad A. Mirkin; C. Shad Thaxton

doi:10.1002/smll.201100566

Hybridization-induced "off-on" ¹⁹F-NMR signal probe release from DNA-functionalized gold nanoparticles

Alexander Kieger, Michael J. Wiester, Daniel Procissi, Todd B. Parrish, Chad A. Mirkin^*, C. Shad Thaxton

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Fluorine-19-DNA-functionalized gold nanoparticles ( ¹⁹F-DNA AuNPs) are demonstrated to provide a ¹⁹F-NMR off-on switch in response to target-specific DNA hybridization events. AuNP-bound ¹⁹F-DNA produces a low intensity signal that is undetectable above background. Upon complementary DNA hybridization and subsequent ¹⁹F-DNA release, the ¹⁹F-NMR signal becomes detectable. This method has the potential to be used both in vitro and in vivo to non-invasively detect and image specific nucleic acid binding events of interest.

Original language	English (US)
Pages (from-to)	1977-1981
Number of pages	5
Journal	Small
Volume	7
Issue number	14
DOIs	https://doi.org/10.1002/smll.201100566
State	Published - Jul 18 2011

Keywords

NMR spectroscopy
fluorine
gold nanoparticles
molecular recognition

ASJC Scopus subject areas

General Chemistry
Biotechnology
General Materials Science
Biomaterials

Access to Document

10.1002/smll.201100566

Cite this

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title = "Hybridization-induced {"}off-on{"} 19F-NMR signal probe release from DNA-functionalized gold nanoparticles",

abstract = "Fluorine-19-DNA-functionalized gold nanoparticles ( 19F-DNA AuNPs) are demonstrated to provide a 19F-NMR off-on switch in response to target-specific DNA hybridization events. AuNP-bound 19F-DNA produces a low intensity signal that is undetectable above background. Upon complementary DNA hybridization and subsequent 19F-DNA release, the 19F-NMR signal becomes detectable. This method has the potential to be used both in vitro and in vivo to non-invasively detect and image specific nucleic acid binding events of interest.",

keywords = "NMR spectroscopy, fluorine, gold nanoparticles, molecular recognition",

author = "Alexander Kieger and Wiester, {Michael J.} and Daniel Procissi and Parrish, {Todd B.} and Mirkin, {Chad A.} and Thaxton, {C. Shad}",

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AU - Kieger, Alexander

AU - Wiester, Michael J.

AU - Procissi, Daniel

AU - Parrish, Todd B.

AU - Mirkin, Chad A.

AU - Thaxton, C. Shad

PY - 2011/7/18

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N2 - Fluorine-19-DNA-functionalized gold nanoparticles ( 19F-DNA AuNPs) are demonstrated to provide a 19F-NMR off-on switch in response to target-specific DNA hybridization events. AuNP-bound 19F-DNA produces a low intensity signal that is undetectable above background. Upon complementary DNA hybridization and subsequent 19F-DNA release, the 19F-NMR signal becomes detectable. This method has the potential to be used both in vitro and in vivo to non-invasively detect and image specific nucleic acid binding events of interest.

AB - Fluorine-19-DNA-functionalized gold nanoparticles ( 19F-DNA AuNPs) are demonstrated to provide a 19F-NMR off-on switch in response to target-specific DNA hybridization events. AuNP-bound 19F-DNA produces a low intensity signal that is undetectable above background. Upon complementary DNA hybridization and subsequent 19F-DNA release, the 19F-NMR signal becomes detectable. This method has the potential to be used both in vitro and in vivo to non-invasively detect and image specific nucleic acid binding events of interest.

KW - NMR spectroscopy

KW - fluorine

KW - gold nanoparticles

KW - molecular recognition

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