Hybridization properties of non-polyadenylated oligo(U)-containing RNA from germinating wheat embryos

M. Dobrzanska; B. Kroczynska; M. Tomaszewski

doi:10.1016/0020-711X(87)90104-2

Hybridization properties of non-polyadenylated oligo(U)-containing RNA from germinating wheat embryos

M. Dobrzanska^*, B. Kroczynska, M. Tomaszewski

^*Corresponding author for this work

Radiation Oncology

Research output: Contribution to journal › Article › peer-review

Abstract

1. 1. Total cytoplasmic RNA of germinating wheat embryos was fractionated by affinity chromatography and separated into non-polyadenylated oligo(U)-containing RNA (A(-)U(+)RNA) and polyadenylated oligo(U)-lacking RNA (A(+)U(-)RNA). 2. 2. The reassociation kinetics of ³²P-labelled complementary DNA (cDNA) reverse-transcribed from A(-)U(+)RNA shows that this RNA fraction is transcribed from unique DNA sequences of the genome similarly as typical mRNA. 3. 3. Cross hybridization experiments show no significant sequence homology between the two RNA fractions. Therefore it is concluded that non-polyadenylated oligo(U)-containing RNA of wheat embryo may represent a discrete class of mRNA.

Original language	English (US)
Pages (from-to)	1205-1210
Number of pages	6
Journal	International Journal of Biochemistry
Volume	19
Issue number	12
DOIs	https://doi.org/10.1016/0020-711X(87)90104-2
State	Published - 1987

Funding

Acknowledgements--We wish to acknowledge the contribution of Professor Jerzy Buchowicz for helpful discussion and criticism of the manuscript. This work was supported by Polish Academy of Sciences within the projects 09.7 and 3.13.

ASJC Scopus subject areas

Biochemistry

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10.1016/0020-711X(87)90104-2

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title = "Hybridization properties of non-polyadenylated oligo(U)-containing RNA from germinating wheat embryos",

abstract = "1. 1. Total cytoplasmic RNA of germinating wheat embryos was fractionated by affinity chromatography and separated into non-polyadenylated oligo(U)-containing RNA (A(-)U(+)RNA) and polyadenylated oligo(U)-lacking RNA (A(+)U(-)RNA). 2. 2. The reassociation kinetics of 32P-labelled complementary DNA (cDNA) reverse-transcribed from A(-)U(+)RNA shows that this RNA fraction is transcribed from unique DNA sequences of the genome similarly as typical mRNA. 3. 3. Cross hybridization experiments show no significant sequence homology between the two RNA fractions. Therefore it is concluded that non-polyadenylated oligo(U)-containing RNA of wheat embryo may represent a discrete class of mRNA.",

author = "M. Dobrzanska and B. Kroczynska and M. Tomaszewski",

note = "Funding Information: Acknowledgements--We wish to acknowledge the contribution of Professor Jerzy Buchowicz for helpful discussion and criticism of the manuscript. This work was supported by Polish Academy of Sciences within the projects 09.7 and 3.13.",

year = "1987",

doi = "10.1016/0020-711X(87)90104-2",

language = "English (US)",

volume = "19",

pages = "1205--1210",

journal = "International Journal of Biochemistry",

issn = "0020-711X",

publisher = "Elsevier Ltd",

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TY - JOUR

T1 - Hybridization properties of non-polyadenylated oligo(U)-containing RNA from germinating wheat embryos

AU - Dobrzanska, M.

AU - Kroczynska, B.

AU - Tomaszewski, M.

N1 - Funding Information: Acknowledgements--We wish to acknowledge the contribution of Professor Jerzy Buchowicz for helpful discussion and criticism of the manuscript. This work was supported by Polish Academy of Sciences within the projects 09.7 and 3.13.

PY - 1987

Y1 - 1987

N2 - 1. 1. Total cytoplasmic RNA of germinating wheat embryos was fractionated by affinity chromatography and separated into non-polyadenylated oligo(U)-containing RNA (A(-)U(+)RNA) and polyadenylated oligo(U)-lacking RNA (A(+)U(-)RNA). 2. 2. The reassociation kinetics of 32P-labelled complementary DNA (cDNA) reverse-transcribed from A(-)U(+)RNA shows that this RNA fraction is transcribed from unique DNA sequences of the genome similarly as typical mRNA. 3. 3. Cross hybridization experiments show no significant sequence homology between the two RNA fractions. Therefore it is concluded that non-polyadenylated oligo(U)-containing RNA of wheat embryo may represent a discrete class of mRNA.

AB - 1. 1. Total cytoplasmic RNA of germinating wheat embryos was fractionated by affinity chromatography and separated into non-polyadenylated oligo(U)-containing RNA (A(-)U(+)RNA) and polyadenylated oligo(U)-lacking RNA (A(+)U(-)RNA). 2. 2. The reassociation kinetics of 32P-labelled complementary DNA (cDNA) reverse-transcribed from A(-)U(+)RNA shows that this RNA fraction is transcribed from unique DNA sequences of the genome similarly as typical mRNA. 3. 3. Cross hybridization experiments show no significant sequence homology between the two RNA fractions. Therefore it is concluded that non-polyadenylated oligo(U)-containing RNA of wheat embryo may represent a discrete class of mRNA.

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JO - International Journal of Biochemistry

JF - International Journal of Biochemistry

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ER -

Hybridization properties of non-polyadenylated oligo(U)-containing RNA from germinating wheat embryos

Abstract

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