1. 1. Total cytoplasmic RNA of germinating wheat embryos was fractionated by affinity chromatography and separated into non-polyadenylated oligo(U)-containing RNA (A(-)U(+)RNA) and polyadenylated oligo(U)-lacking RNA (A(+)U(-)RNA). 2. 2. The reassociation kinetics of 32P-labelled complementary DNA (cDNA) reverse-transcribed from A(-)U(+)RNA shows that this RNA fraction is transcribed from unique DNA sequences of the genome similarly as typical mRNA. 3. 3. Cross hybridization experiments show no significant sequence homology between the two RNA fractions. Therefore it is concluded that non-polyadenylated oligo(U)-containing RNA of wheat embryo may represent a discrete class of mRNA.
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