Hybridoma antibody content and production rate in continuous culture: Effect of dilution rate

Sridhar Reddy*, William M Miller

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Hybridoma IND1 viability was ≥95% for dilution rates (D) ranging from 45 to 100% of μmax (0.037 hr-1). Over this range, the cell concentration and total protein content increased with D. Washout occurred at D=0.041 hr-1, but the intracellular protein content continued to increase. The high- and low-content modes of the intracellular antibody distribution did not vary with D. The fraction of cells with high antibody content decreased with time, except for an increase at D=0.041 hr-1. This decrease did not affect the specific antibody production rate, which, like the high- and low-content modes, was independent of D.

Original languageEnglish (US)
Pages (from-to)1007-1012
Number of pages6
JournalBiotechnology Letters
Volume14
Issue number11
DOIs
StatePublished - Nov 1 1992

ASJC Scopus subject areas

  • Biotechnology

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