Abstract
Hybridoma IND1 viability was ≥95% for dilution rates (D) ranging from 45 to 100% of μmax (0.037 hr-1). Over this range, the cell concentration and total protein content increased with D. Washout occurred at D=0.041 hr-1, but the intracellular protein content continued to increase. The high- and low-content modes of the intracellular antibody distribution did not vary with D. The fraction of cells with high antibody content decreased with time, except for an increase at D=0.041 hr-1. This decrease did not affect the specific antibody production rate, which, like the high- and low-content modes, was independent of D.
Original language | English (US) |
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Pages (from-to) | 1007-1012 |
Number of pages | 6 |
Journal | Biotechnology Letters |
Volume | 14 |
Issue number | 11 |
DOIs | |
State | Published - Nov 1 1992 |
ASJC Scopus subject areas
- Biotechnology