Hyposialylation of integrins stimulates the activity of myeloid fibronectin receptors

Alexis C. Semel, Eric C. Seales, Anuj Singhal, Elizabeth A. Eklund, Karen J. Colley, Susan L. Bellis*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

88 Scopus citations


Despite numerous reports suggesting that β1, integrin receptors undergo differential glycosylation, the potential role of N-linked carbohydrates in modulating integrin function has been largely ignored. In the present study, we find that β1 integrins are differentially glycosylated during phorbol ester (PMA)-stimulated differentiation of myeloid cells along the monocyte/macrophage lineage. PMA treatment of two myeloid cell lines, U937 and THP-1, induces a down-regulation in expression of the ST6Gal I sialyltransferase. Correspondingly, the β1 integrin subunit becomes hyposialylated, suggesting that the β1 integrin is a substrate for this enzyme. The expression of hyposialylated β1 integrin isoforms is temporally correlated with enhanced binding of myeloid cells to fibronectin, and, importantly, fibronectin binding is inhibited when the Golgi disrupter, brefeldin A, is used to block the expression of the hyposialylated form. Consistent with the observation that cells with hyposialylated integrins are more adhesive to fibronectin, we demonstrate that the enzymatic removal of sialic acid residues from purified α5β1 integrins stimulates fibronectin binding by these integrins. These data support the hypothesis that unsialylated β1 integrins are more adhesive to fibronectin, although desialylation of α5 subunits could also contribute to increased fibronectin binding. Collectively our results suggest a novel mechanism for regulation of the β1 integrin family of cell adhesion receptors.

Original languageEnglish (US)
Pages (from-to)32830-32836
Number of pages7
JournalJournal of Biological Chemistry
Issue number36
StatePublished - Sep 6 2002

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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