Hyposmotic swelling induces magnitude and gain change in the electromotile performance of isolated outer hair cells

Istvan Sziklai*, Peter Dallos

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

OHCs were inserted into a partitioning microchamber. Reversible swelling was induced by hyposmotic incubation medium (270 mosm.kg H2O-1). The transfer curve of electromotility was measured by stimulating the OHCs with a series of brief (2.5 ms) square-pulses with both polarities and graded magnitudes. Response magnitude and gain of electromotility were increased in swollen OHCs. On the basis of their electromotile performance-characteristics, swelling appeared to depolarize the OHCs. Transition from contraction dominated asymmetry of motility was observed towards a symmetric response. Depolarization, by itself, cannot explain the gain and magnitude increase. Acetylcholine (ACh) responsiveness of swollen OHCs preserves the pre-challenged pattern: ACh further increases gain and magnitude.

Original languageEnglish (US)
Pages (from-to)222-225
Number of pages4
JournalActa Oto-Laryngologica
Volume117
Issue number2
DOIs
StatePublished - Jan 1 1997

Keywords

  • acetylcholine
  • electromotility
  • hyposmotic challenge
  • isolated outer hair cells
  • swelling

ASJC Scopus subject areas

  • Otorhinolaryngology

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