Hyposmotic swelling induces magnitude and gain change in the electromotile performance of isolated outer hair cells

Istvan Sziklai; Peter Dallos

doi:10.3109/00016489709117775

Hyposmotic swelling induces magnitude and gain change in the electromotile performance of isolated outer hair cells

Istvan Sziklai^*, Peter Dallos

^*Corresponding author for this work

Communication Sciences and Disorders

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

OHCs were inserted into a partitioning microchamber. Reversible swelling was induced by hyposmotic incubation medium (270 mosm.kg H₂O^-1). The transfer curve of electromotility was measured by stimulating the OHCs with a series of brief (2.5 ms) square-pulses with both polarities and graded magnitudes. Response magnitude and gain of electromotility were increased in swollen OHCs. On the basis of their electromotile performance-characteristics, swelling appeared to depolarize the OHCs. Transition from contraction dominated asymmetry of motility was observed towards a symmetric response. Depolarization, by itself, cannot explain the gain and magnitude increase. Acetylcholine (ACh) responsiveness of swollen OHCs preserves the pre-challenged pattern: ACh further increases gain and magnitude.

Original language	English (US)
Pages (from-to)	222-225
Number of pages	4
Journal	Acta Oto-Laryngologica
Volume	117
Issue number	2
DOIs	https://doi.org/10.3109/00016489709117775
State	Published - 1997

Funding

This work was supported by an NIDCD grant DC 00708 and OTKA grant (National Scientific Research Council of Hungary) No. T 014 753.

Keywords

acetylcholine
electromotility
hyposmotic challenge
isolated outer hair cells
swelling

ASJC Scopus subject areas

Otorhinolaryngology

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10.3109/00016489709117775

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title = "Hyposmotic swelling induces magnitude and gain change in the electromotile performance of isolated outer hair cells",

abstract = "OHCs were inserted into a partitioning microchamber. Reversible swelling was induced by hyposmotic incubation medium (270 mosm.kg H2O-1). The transfer curve of electromotility was measured by stimulating the OHCs with a series of brief (2.5 ms) square-pulses with both polarities and graded magnitudes. Response magnitude and gain of electromotility were increased in swollen OHCs. On the basis of their electromotile performance-characteristics, swelling appeared to depolarize the OHCs. Transition from contraction dominated asymmetry of motility was observed towards a symmetric response. Depolarization, by itself, cannot explain the gain and magnitude increase. Acetylcholine (ACh) responsiveness of swollen OHCs preserves the pre-challenged pattern: ACh further increases gain and magnitude.",

keywords = "acetylcholine, electromotility, hyposmotic challenge, isolated outer hair cells, swelling",

author = "Istvan Sziklai and Peter Dallos",

note = "Funding Information: This work was supported by an NIDCD grant DC 00708 and OTKA grant (National Scientific Research Council of Hungary) No. T 014 753.",

year = "1997",

doi = "10.3109/00016489709117775",

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journal = "Acta Oto-Laryngologica",

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AU - Sziklai, Istvan

AU - Dallos, Peter

N1 - Funding Information: This work was supported by an NIDCD grant DC 00708 and OTKA grant (National Scientific Research Council of Hungary) No. T 014 753.

PY - 1997

Y1 - 1997

N2 - OHCs were inserted into a partitioning microchamber. Reversible swelling was induced by hyposmotic incubation medium (270 mosm.kg H2O-1). The transfer curve of electromotility was measured by stimulating the OHCs with a series of brief (2.5 ms) square-pulses with both polarities and graded magnitudes. Response magnitude and gain of electromotility were increased in swollen OHCs. On the basis of their electromotile performance-characteristics, swelling appeared to depolarize the OHCs. Transition from contraction dominated asymmetry of motility was observed towards a symmetric response. Depolarization, by itself, cannot explain the gain and magnitude increase. Acetylcholine (ACh) responsiveness of swollen OHCs preserves the pre-challenged pattern: ACh further increases gain and magnitude.

AB - OHCs were inserted into a partitioning microchamber. Reversible swelling was induced by hyposmotic incubation medium (270 mosm.kg H2O-1). The transfer curve of electromotility was measured by stimulating the OHCs with a series of brief (2.5 ms) square-pulses with both polarities and graded magnitudes. Response magnitude and gain of electromotility were increased in swollen OHCs. On the basis of their electromotile performance-characteristics, swelling appeared to depolarize the OHCs. Transition from contraction dominated asymmetry of motility was observed towards a symmetric response. Depolarization, by itself, cannot explain the gain and magnitude increase. Acetylcholine (ACh) responsiveness of swollen OHCs preserves the pre-challenged pattern: ACh further increases gain and magnitude.

KW - acetylcholine

KW - electromotility

KW - hyposmotic challenge

KW - isolated outer hair cells

KW - swelling

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Hyposmotic swelling induces magnitude and gain change in the electromotile performance of isolated outer hair cells

Abstract

Funding

Keywords

ASJC Scopus subject areas

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