Identification and analysis of expressed sequence tags related to K562 cells into erythroid differentiation

Jia Yu*, Jun wu Zhang, Han Peng, Deng Chen

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To isolate expressed sequence tags (ESTs) related to K562 cells erythroid differentiation. METHODS: Modified differential display reverse transcription polymerase chain reaction (DDRT-PCR) method was applied to identify differential ESTs in uninduced and induced K562 cells by HEMIN for 36 hours. Remarkable differential ESTs were firstly selected for cloning, sequencing and bioinformational analyzing. Several ESTs representing new sequence or providing functional clue were selected for Northern blot analysis. RESULTS: Sixty differentially expressed cDNA fragments related to K562 cells inducted into erythroid differentiation by HEMIN were obtained. Among them, 38 were upregulated and 22 downregulated. Among the 40 differential ESTs selected for cloning, sequencing and bioinformationally analyzing, 23 were found to match to known GenBank sequences and 10 represented cDNA sequences with only dbEST database matches and 7 ESTs have no any database matches. The results of 6 in 8 ESTs selected for Northern blot analysis were shown to be consistent with the differential expressions of DDRT-PCR. CONCLUSIONS: The improved DDRT-PCR method had successfully overcome the problem of false positive. These ESTs provide some clue for studying the molecular mechanisms and regulation network of erythroid differentiation.

Original languageEnglish (US)
Pages (from-to)150-154
Number of pages5
JournalZhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
Volume26
Issue number2
StatePublished - Apr 2004

ASJC Scopus subject areas

  • Medicine(all)

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