Identification and characterization of a Neisseria gonorrhoeae gene encoding a glycolipid-binding adhesin

Durga K. Paruchuri*, H. Steven Seifert, Richard S. Ajioka, Karl Anders Karlsson, Magdalene So

*Corresponding author for this work

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

We recently identified a set of mammalian cell receptors for Neisseria gonorrhoeae that are glycolipids. These receptors, lactosylceramide [Gal(β1-4)Glc(β1-l)Cer], gangliotriosylceramide [GalNAc(β1-4)Gal(β1-4)Glc(β1-1)Cer], and gangliotetraosylceramide [Gal(β1-3)GalNAc(β1-4)Gal(β1-4)Glc(β1-1)Cer], were shown to be specifically bound by a gonococcal outer membrane protein distinct from pilin and protein II. Here we report the isolation of the gene encoding the gangliotetraosylceramide-binding adhesin from a N. gonorrhoeae MS11 gene bank in Escherichia coli. Transposon mutagenesis studies in E. coli indicate that the adhesin is a protein with a molecular mass of 36,000 Da. The gene encoding the 36-kDa protein is duplicated in MS11 since two transposon insertions were required to abolish expression of the gene in this bacterium. This protein is present on the surface of the gonococcus and is not associated with the pilus.

Original languageEnglish (US)
Pages (from-to)333-337
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number1
DOIs
StatePublished - 1990

Keywords

  • Adhesion
  • Bacterial binding
  • Overlay assay
  • Receptors

ASJC Scopus subject areas

  • General

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