Identification and characterization of suppressor of cytokine signaling 1 (SOCS-1) homologues in teleost fish

Hong Jian Jin, Li Xin Xiang, Jian Zhong Shao*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    33 Scopus citations


    The suppressor of cytokine signaling 1 (SOCS-1), as the name implies, is a member of proteins that regulate cytokine signaling pathways by inhibiting the events of key tyrosine phosphorylation on cytokine receptors and signaling molecules such as Janus kinase family members. Although mammalian SOCS-1 homologues were characterized in several species, no similar research work has been reported in fish yet. In this paper, we initially cloned the SOCS-1 genes from Tetraodon nigroviridis and Danio rerio, and identified other two SOCS-1 genes from Fugu rubripes and Gasterosteus aculeatus. The results showed that the fish SOCS-1-encoding genes consist of two exons and a single intron, a typical characteristic of SOCS family in gene organization. Moreover, two alternatively spliced transcripts that encoded 220 and 196 amino acids were obtained in T. nigroviridis, proving the distinct existence of alternative splicing in fish SOCS-1 different from higher vertebrates. By reverse transcriptase polymerase chain reaction (PCR) and real-time quantitative PCR, gene expression studies indicated that both two alternatively spliced transcripts of Tetraodon SOCS-1 were expressed extensively in major tissues as we examined and their corresponding expression levels could be strikingly raised at 3 h postinjection with lipopolysaccharide, which strongly suggested that SOCS-1 proteins in fish might be involved in inflammatory responses. This is the first report of cloning and characterization of SOCS-1 complementary deoxyribonucleic acids and genes in fish.

    Original languageEnglish (US)
    Pages (from-to)673-686
    Number of pages14
    Issue number8
    StatePublished - Aug 1 2007


    • Alternative splicing
    • Cytokine signaling
    • Danio rerio
    • Fugu rubripes
    • Gasterosteus aculeatus
    • SOCS-1
    • Tetraodon nigroviridis

    ASJC Scopus subject areas

    • Immunology
    • Genetics

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