Identification and molecular characterization of CALM/AF10 fusion products in T cell acute lymphoblastic leukemia and acute myeloid leukemia

K. M. Carlson; C. Vignon; S. Bohlander; J. A. Martinez-Climent; M. M. Le Beau; J. D. Rowley

doi:10.1038/sj.leu.2401629

Identification and molecular characterization of CALM/AF10 fusion products in T cell acute lymphoblastic leukemia and acute myeloid leukemia

K. M. Carlson, C. Vignon, S. Bohlander, J. A. Martinez-Climent, M. M. Le Beau, J. D. Rowley^*

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

The t(10;11)(p12-p13;q14-q21) observed in a subset of patients with either acute lymphoblastic leukemia or acute myeloid leukemia has been shown to result in the fusion of AF10 on chromosome 10 with CALM (also named CLTH) on chromosome 11. AF10 was originally identified as a fusion partner of MLL in the t(10;11)(p12-p13;q23) observed in myeloid leukemia. CALM is a newly isolated gene, cloned as the fusion partner of AF10 in the monocytoid cell line, U937. In order to understand the relationship between MLL, AF10, CALM and the leukemic process, fluorescence in situ hybridization and reverse transcriptase polymerase chain reaction were used to study a series of nine leukemia patients with a t(10;11). Six had myeloid leukemia (AML-M0, AML-M1, AML-M4 and AML-M5) and three had T cell lymphoblastic leukemia. We identified four different CALM/AF10 fusion products in five patients and AF10/CALM reciprocal message in one. We conclude that fusion of CALM and AF10 is a recurring abnormality in both lymphoid and myeloid leukemias of various types including AML-MS, and that the breakpoints in the two types of leukemia do not differ. Our data indicate that the CALM/AF10 fusion product on the der(10) chromosome is critical to leukemogenesis.

Original language	English (US)
Pages (from-to)	100-104
Number of pages	5
Journal	Leukemia
Volume	14
Issue number	1
DOIs	https://doi.org/10.1038/sj.leu.2401629
State	Published - 2000

Keywords

AF10
Acute lymphoid leukemia
Acute myeloid leukemia
CALM
T(10;11)
Translocations

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/sj.leu.2401629

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@article{add7e7bbaa584b92a92bcaa9ce6cf880,

title = "Identification and molecular characterization of CALM/AF10 fusion products in T cell acute lymphoblastic leukemia and acute myeloid leukemia",

abstract = "The t(10;11)(p12-p13;q14-q21) observed in a subset of patients with either acute lymphoblastic leukemia or acute myeloid leukemia has been shown to result in the fusion of AF10 on chromosome 10 with CALM (also named CLTH) on chromosome 11. AF10 was originally identified as a fusion partner of MLL in the t(10;11)(p12-p13;q23) observed in myeloid leukemia. CALM is a newly isolated gene, cloned as the fusion partner of AF10 in the monocytoid cell line, U937. In order to understand the relationship between MLL, AF10, CALM and the leukemic process, fluorescence in situ hybridization and reverse transcriptase polymerase chain reaction were used to study a series of nine leukemia patients with a t(10;11). Six had myeloid leukemia (AML-M0, AML-M1, AML-M4 and AML-M5) and three had T cell lymphoblastic leukemia. We identified four different CALM/AF10 fusion products in five patients and AF10/CALM reciprocal message in one. We conclude that fusion of CALM and AF10 is a recurring abnormality in both lymphoid and myeloid leukemias of various types including AML-MS, and that the breakpoints in the two types of leukemia do not differ. Our data indicate that the CALM/AF10 fusion product on the der(10) chromosome is critical to leukemogenesis.",

keywords = "AF10, Acute lymphoid leukemia, Acute myeloid leukemia, CALM, T(10;11), Translocations",

author = "Carlson, {K. M.} and C. Vignon and S. Bohlander and Martinez-Climent, {J. A.} and {Le Beau}, {M. M.} and Rowley, {J. D.}",

note = "Funding Information: We are grateful to Marjorie Isaacson for retrieval of patient information and data management, and to Dr Carlos Suarez, Loyola University Medical Center, for referral of patient 5. This work was supported in part by the National Cancer Institute, CA 42557 (JDR). KC was supported by the G Harold and Leila Y Mathers Charitable Foundation.",

year = "2000",

doi = "10.1038/sj.leu.2401629",

language = "English (US)",

volume = "14",

pages = "100--104",

journal = "Leukemia",

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T1 - Identification and molecular characterization of CALM/AF10 fusion products in T cell acute lymphoblastic leukemia and acute myeloid leukemia

AU - Carlson, K. M.

AU - Vignon, C.

AU - Bohlander, S.

AU - Martinez-Climent, J. A.

AU - Le Beau, M. M.

AU - Rowley, J. D.

N1 - Funding Information: We are grateful to Marjorie Isaacson for retrieval of patient information and data management, and to Dr Carlos Suarez, Loyola University Medical Center, for referral of patient 5. This work was supported in part by the National Cancer Institute, CA 42557 (JDR). KC was supported by the G Harold and Leila Y Mathers Charitable Foundation.

PY - 2000

Y1 - 2000

N2 - The t(10;11)(p12-p13;q14-q21) observed in a subset of patients with either acute lymphoblastic leukemia or acute myeloid leukemia has been shown to result in the fusion of AF10 on chromosome 10 with CALM (also named CLTH) on chromosome 11. AF10 was originally identified as a fusion partner of MLL in the t(10;11)(p12-p13;q23) observed in myeloid leukemia. CALM is a newly isolated gene, cloned as the fusion partner of AF10 in the monocytoid cell line, U937. In order to understand the relationship between MLL, AF10, CALM and the leukemic process, fluorescence in situ hybridization and reverse transcriptase polymerase chain reaction were used to study a series of nine leukemia patients with a t(10;11). Six had myeloid leukemia (AML-M0, AML-M1, AML-M4 and AML-M5) and three had T cell lymphoblastic leukemia. We identified four different CALM/AF10 fusion products in five patients and AF10/CALM reciprocal message in one. We conclude that fusion of CALM and AF10 is a recurring abnormality in both lymphoid and myeloid leukemias of various types including AML-MS, and that the breakpoints in the two types of leukemia do not differ. Our data indicate that the CALM/AF10 fusion product on the der(10) chromosome is critical to leukemogenesis.

AB - The t(10;11)(p12-p13;q14-q21) observed in a subset of patients with either acute lymphoblastic leukemia or acute myeloid leukemia has been shown to result in the fusion of AF10 on chromosome 10 with CALM (also named CLTH) on chromosome 11. AF10 was originally identified as a fusion partner of MLL in the t(10;11)(p12-p13;q23) observed in myeloid leukemia. CALM is a newly isolated gene, cloned as the fusion partner of AF10 in the monocytoid cell line, U937. In order to understand the relationship between MLL, AF10, CALM and the leukemic process, fluorescence in situ hybridization and reverse transcriptase polymerase chain reaction were used to study a series of nine leukemia patients with a t(10;11). Six had myeloid leukemia (AML-M0, AML-M1, AML-M4 and AML-M5) and three had T cell lymphoblastic leukemia. We identified four different CALM/AF10 fusion products in five patients and AF10/CALM reciprocal message in one. We conclude that fusion of CALM and AF10 is a recurring abnormality in both lymphoid and myeloid leukemias of various types including AML-MS, and that the breakpoints in the two types of leukemia do not differ. Our data indicate that the CALM/AF10 fusion product on the der(10) chromosome is critical to leukemogenesis.

KW - AF10

KW - Acute lymphoid leukemia

KW - Acute myeloid leukemia

KW - CALM

KW - T(10;11)

KW - Translocations

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M3 - Article

C2 - 10637483

AN - SCOPUS:0033978155

SN - 0887-6924

VL - 14

SP - 100

EP - 104

JO - Leukemia

JF - Leukemia

IS - 1

ER -

Identification and molecular characterization of CALM/AF10 fusion products in T cell acute lymphoblastic leukemia and acute myeloid leukemia

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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