@article{765e29d2dd974668a2ac49c379fef5b9,
title = "Identification and Quantification of Proteoforms by Mass Spectrometry",
abstract = "A proteoform is a defined form of a protein derived from a given gene with a specific amino acid sequence and localized post-translational modifications. In top-down proteomic analyses, proteoforms are identified and quantified through mass spectrometric analysis of intact proteins. Recent technological developments have enabled comprehensive proteoform analyses in complex samples, and an increasing number of laboratories are adopting top-down proteomic workflows. In this review, some recent advances are outlined and current challenges and future directions for the field are discussed.",
keywords = "bioinformatics, mass spectrometry, proteoform, proteoform family, top-down proteomics",
author = "Schaffer, {Leah V.} and Millikin, {Robert J.} and Miller, {Rachel M.} and Anderson, {Lissa C.} and Fellers, {Ryan T.} and Ying Ge and Kelleher, {Neil L.} and LeDuc, {Richard D.} and Xiaowen Liu and Payne, {Samuel H.} and Liangliang Sun and Thomas, {Paul M.} and Trisha Tucholski and Zhe Wang and Si Wu and Zhijie Wu and Dahang Yu and Shortreed, {Michael R.} and Smith, {Lloyd M.}",
note = "Funding Information: This work was funded by the National Institute of General Medical Sciences of the National Institutes of Health (NIH) under Award Numbers R35GM126914 and R01GM114292 (to L.M.S.). L.V.S was supported by the NIH Biotechnology Training Program, T32GM008349. R.J.M was supported by the NIH Genomic Sciences Training Program, 5T32HG002760. R.M.M and T.T. were supported by the NIH Chemistry Biology Interface Training Program, T32GM008505. L.S. was supported by the NIH Grant R01GM125991. X.L. was supported by NIH Grants R01GM118470 and R01GM125991. Z.W. was supported by R01 GM117058, R01 GM125085. Y.G. would like to acknowledge NIH grants, R01HL109810, R01HL096971, R01GM117058, R01GM125085, and S10OD018475. L.C.A was supported by NSF/DMR-1644779. R.T.F., N.L.K., R.D.L., and P.M.T. acknowledge P41GM108569. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. Publisher Copyright: {\textcopyright} 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2019",
month = may,
doi = "10.1002/pmic.201800361",
language = "English (US)",
volume = "19",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "10",
}