Identification and Quantification of Proteoforms by Mass Spectrometry

Leah V. Schaffer, Robert J. Millikin, Rachel M. Miller, Lissa C. Anderson, Ryan T. Fellers, Ying Ge, Neil L. Kelleher, Richard D. LeDuc, Xiaowen Liu, Samuel H. Payne, Liangliang Sun, Paul M. Thomas, Trisha Tucholski, Zhe Wang, Si Wu, Zhijie Wu, Dahang Yu, Michael R. Shortreed, Lloyd M. Smith*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

60 Scopus citations

Abstract

A proteoform is a defined form of a protein derived from a given gene with a specific amino acid sequence and localized post-translational modifications. In top-down proteomic analyses, proteoforms are identified and quantified through mass spectrometric analysis of intact proteins. Recent technological developments have enabled comprehensive proteoform analyses in complex samples, and an increasing number of laboratories are adopting top-down proteomic workflows. In this review, some recent advances are outlined and current challenges and future directions for the field are discussed.

Original languageEnglish (US)
Article number1800361
JournalProteomics
Volume19
Issue number10
DOIs
StatePublished - May 2019

Keywords

  • bioinformatics
  • mass spectrometry
  • proteoform
  • proteoform family
  • top-down proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Identification and Quantification of Proteoforms by Mass Spectrometry'. Together they form a unique fingerprint.

Cite this