Identification of a human erythroid progenitor cell population which expresses the CD34 antigen and binds the plant lectin Ulex europaeus I

Kristen L. Unverzagt, Jeffrey Martinson, Wanda Lee, Patrick J. Stiff, Stephanie Williams, James G. Bender*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Two and three color flow cytometry of normal human bone marrow was used to identify CD34+ progenitor cells and examine their binding to the plant lectin Ulex europaeus I (Ulex). In normal bone marrow, 48.48 ± 17.4% of the CD34+ cells bind to Ulex. Two color flow cytometry was used to sort CD34+ cells, and subsets of CD34+ cells, CD34+ Ulex+ and CD34+ Ulex-. These populations were sorted into colony assays to assess myeloid (CFU-GM) and erythroid (BFU-E) progenitors. The CD34+ Ulex+ subset was 84 ± 14% BFU-E colonies (mean ± S.D.) and had the highest cloning efficiency of 28 ± 13%. Three color analysis of CD34+ Ulex+ cells showed staining with other erythroid (CD71, GlyA) antibodies and lack of staining with myeloid (CD13, CD45RA) antibodies. These studies confirmed the erythroid characteristics of this subpopulation.

Original languageEnglish (US)
Pages (from-to)54-58
Number of pages5
JournalCytometry
Volume23
Issue number1
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Keywords

  • CD34
  • colony forming cells
  • erythroid progenitors
  • hematopoiesis
  • lectin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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