Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast

François Périer; Kathryn L. Coulter; Hong Liang; Carolyn M. Radeke; Richard F. Gaber; Carol A. Vandenberg

doi:10.1016/0014-5793(94)00879-5

Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast

François Périer, Kathryn L. Coulter, Hong Liang, Carolyn M. Radeke, Richard F. Gaber, Carol A. Vandenberg^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Two suppressors of the growth deficiency of a potassium transport mutant of Saccharomyces cerevisiae were isolated from a mouse cDNA expression library. These suppressors, SKD1 and SKD2 (suppressor of K⁺ transport growth defect), were cDNAs encoding members of a family of ATPases involved in membrane fusion (N-ethylmaleimide-sensitive fusion protein, NSF), cell division cycle regulation (CDC48p), peroxisome assembly (Pas1p), and transcriptional regulation (TBP-1). The SKD1 protein constitutes a novel member of this family with 49-58% amino acid sequence similarity with other family members, and contains a single ATP binding site. The SKD2 polypeptide is the mouse homolog of NSF.

Original language	English (US)
Pages (from-to)	286-290
Number of pages	5
Journal	FEBS Letters
Volume	351
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(94)00879-5
State	Published - Sep 5 1994

Keywords

ATPase
Membrane fusion
Potassium transport
Suppressor
TRK
Yeast

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast",

abstract = "Two suppressors of the growth deficiency of a potassium transport mutant of Saccharomyces cerevisiae were isolated from a mouse cDNA expression library. These suppressors, SKD1 and SKD2 (suppressor of K+ transport growth defect), were cDNAs encoding members of a family of ATPases involved in membrane fusion (N-ethylmaleimide-sensitive fusion protein, NSF), cell division cycle regulation (CDC48p), peroxisome assembly (Pas1p), and transcriptional regulation (TBP-1). The SKD1 protein constitutes a novel member of this family with 49-58% amino acid sequence similarity with other family members, and contains a single ATP binding site. The SKD2 polypeptide is the mouse homolog of NSF.",

keywords = "ATPase, Membrane fusion, Potassium transport, Suppressor, TRK, Yeast",

author = "Fran{\c c}ois P{\'e}rier and Coulter, {Kathryn L.} and Hong Liang and Radeke, {Carolyn M.} and Gaber, {Richard F.} and Vandenberg, {Carol A.}",

note = "Funding Information: Acknowledgements. This work was supported by grants from the National Institutes of Health (HL41656) and the American Heart Association, California Affiliate, to C.A.V., from the National Institutes of Health (IR01GM45739) and the National Science Foundation (DCB-9012213) to R.F.G., and by a National Multiple Sclerosis postdoctoral fellowship to F.R",

year = "1994",

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doi = "10.1016/0014-5793(94)00879-5",

language = "English (US)",

volume = "351",

pages = "286--290",

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T1 - Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast

AU - Périer, François

AU - Coulter, Kathryn L.

AU - Liang, Hong

AU - Radeke, Carolyn M.

AU - Gaber, Richard F.

AU - Vandenberg, Carol A.

N1 - Funding Information: Acknowledgements. This work was supported by grants from the National Institutes of Health (HL41656) and the American Heart Association, California Affiliate, to C.A.V., from the National Institutes of Health (IR01GM45739) and the National Science Foundation (DCB-9012213) to R.F.G., and by a National Multiple Sclerosis postdoctoral fellowship to F.R

PY - 1994/9/5

Y1 - 1994/9/5

N2 - Two suppressors of the growth deficiency of a potassium transport mutant of Saccharomyces cerevisiae were isolated from a mouse cDNA expression library. These suppressors, SKD1 and SKD2 (suppressor of K+ transport growth defect), were cDNAs encoding members of a family of ATPases involved in membrane fusion (N-ethylmaleimide-sensitive fusion protein, NSF), cell division cycle regulation (CDC48p), peroxisome assembly (Pas1p), and transcriptional regulation (TBP-1). The SKD1 protein constitutes a novel member of this family with 49-58% amino acid sequence similarity with other family members, and contains a single ATP binding site. The SKD2 polypeptide is the mouse homolog of NSF.

AB - Two suppressors of the growth deficiency of a potassium transport mutant of Saccharomyces cerevisiae were isolated from a mouse cDNA expression library. These suppressors, SKD1 and SKD2 (suppressor of K+ transport growth defect), were cDNAs encoding members of a family of ATPases involved in membrane fusion (N-ethylmaleimide-sensitive fusion protein, NSF), cell division cycle regulation (CDC48p), peroxisome assembly (Pas1p), and transcriptional regulation (TBP-1). The SKD1 protein constitutes a novel member of this family with 49-58% amino acid sequence similarity with other family members, and contains a single ATP binding site. The SKD2 polypeptide is the mouse homolog of NSF.

KW - ATPase

KW - Membrane fusion

KW - Potassium transport

KW - Suppressor

KW - TRK

KW - Yeast

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Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast

Abstract

Keywords

ASJC Scopus subject areas

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