Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast

François Périer, Kathryn L. Coulter, Hong Liang, Carolyn M. Radeke, Richard F. Gaber, Carol A. Vandenberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Two suppressors of the growth deficiency of a potassium transport mutant of Saccharomyces cerevisiae were isolated from a mouse cDNA expression library. These suppressors, SKD1 and SKD2 (suppressor of K+ transport growth defect), were cDNAs encoding members of a family of ATPases involved in membrane fusion (N-ethylmaleimide-sensitive fusion protein, NSF), cell division cycle regulation (CDC48p), peroxisome assembly (Pas1p), and transcriptional regulation (TBP-1). The SKD1 protein constitutes a novel member of this family with 49-58% amino acid sequence similarity with other family members, and contains a single ATP binding site. The SKD2 polypeptide is the mouse homolog of NSF.

Original languageEnglish (US)
Pages (from-to)286-290
Number of pages5
JournalFEBS Letters
Volume351
Issue number2
DOIs
StatePublished - Sep 5 1994

Funding

Acknowledgements. This work was supported by grants from the National Institutes of Health (HL41656) and the American Heart Association, California Affiliate, to C.A.V., from the National Institutes of Health (IR01GM45739) and the National Science Foundation (DCB-9012213) to R.F.G., and by a National Multiple Sclerosis postdoctoral fellowship to F.R

Keywords

  • ATPase
  • Membrane fusion
  • Potassium transport
  • Suppressor
  • TRK
  • Yeast

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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