Identification of a protein interacting with apoptin from human leucocyte cDNA library by using yeast two-hybrid screening

Guo Jing Sun, Xin Tong, Yan Dong, Zhu Zhong Mei, Zhi Xian Sun*

*Corresponding author for this work

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

To screen the protein interacting with apoptin from human leucocyte cDNA library by using yeast two-hybrid system, four clones interacting with apoptin were identified. One of them was homologous with Nmi (N-Myc interaction protein). Cell co-immunoprecipitation showed that apoptin could bind to Nmi in mammalian cells. Apoptin mutants T1, T2 and T3 lacked the C-terminal 11 AA, 33-46 AA and both, respectively. Apoptin mutants T2 and T3 failed to interact with Nmi, suggesting that its 33-46 AA was pivotal for the interaction. Apoptin mutant T1 still interacted with Nmi, suggesting that its C-terminal 11 AA was not essential for the interaction.

Original languageEnglish (US)
Pages (from-to)369-372
Number of pages4
JournalActa biochimica et biophysica Sinica
Volume34
Issue number3
StatePublished - 2002

Keywords

  • Apoptin
  • Co-immunoprecipitation
  • Nmi
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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