Abstract
In recent years multi-parameter flow cytometry has enabled identification of cells at major stages in myeloid development; from pluripotent hematopoietic stem cells, through populations with increasingly limited developmental potential (common myeloid progenitors and granulocyte-macrophage progenitors), to terminally differentiated mature cells. Myeloid progenitors are heterogeneous, and the surface markers that define transition states from progenitors to mature cells are poorly characterized. Siglec-F is a surface glycoprotein frequently used in combination with IL-5 receptor alpha (IL5Rα) for the identification of murine eosinophils. Here, we describe a CD11b+ Siglec-F+ IL5Rα− myeloid population in the bone marrow of C57BL/6 mice. The CD11b+ Siglec-F+ IL5Rα− cells are retained in eosinophil deficient PHIL mice, and are not expanded upon overexpression of IL-5, indicating that they are upstream or independent of the eosinophil lineage. We show these cells to have GMP-like developmental potential in vitro and in vivo, and to be transcriptionally distinct from the classically described GMP population. The CD11b+ Siglec-F+ IL5Rα− population expands in the bone marrow of Myb mutant mice, which is potentially due to negative transcriptional regulation of Siglec-F by Myb. Lastly, we show that the role of Siglec-F may be, at least in part, to regulate GMP viability.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 123-133 |
| Number of pages | 11 |
| Journal | Journal of Leukocyte Biology |
| Volume | 104 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1 2018 |
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Keywords
- Myb
- eosinophil
- granulocyte
- hematopoiesis
- neutrophil
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Cell Biology
Cite this
}
Identification of a Siglec-F+ granulocyte-macrophage progenitor. / Bolden, Jessica E.; Lucas, Erin C.; Zhou, Geyu; O'Sullivan, Jeremy Alastair; de Graaf, Carolyn A.; McKenzie, Mark D.; Di Rago, Ladina; Baldwin, Tracey M.; Shortt, Jake; Alexander, Warren S.; Bochner, Bruce Scott; Ritchie, Matthew E.; Hilton, Douglas J.; Fairfax, Kirsten A.
In: Journal of Leukocyte Biology, Vol. 104, No. 1, 01.07.2018, p. 123-133.Research output: Contribution to journal › Article
TY - JOUR
T1 - Identification of a Siglec-F+ granulocyte-macrophage progenitor
AU - Bolden, Jessica E.
AU - Lucas, Erin C.
AU - Zhou, Geyu
AU - O'Sullivan, Jeremy Alastair
AU - de Graaf, Carolyn A.
AU - McKenzie, Mark D.
AU - Di Rago, Ladina
AU - Baldwin, Tracey M.
AU - Shortt, Jake
AU - Alexander, Warren S.
AU - Bochner, Bruce Scott
AU - Ritchie, Matthew E.
AU - Hilton, Douglas J.
AU - Fairfax, Kirsten A.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - In recent years multi-parameter flow cytometry has enabled identification of cells at major stages in myeloid development; from pluripotent hematopoietic stem cells, through populations with increasingly limited developmental potential (common myeloid progenitors and granulocyte-macrophage progenitors), to terminally differentiated mature cells. Myeloid progenitors are heterogeneous, and the surface markers that define transition states from progenitors to mature cells are poorly characterized. Siglec-F is a surface glycoprotein frequently used in combination with IL-5 receptor alpha (IL5Rα) for the identification of murine eosinophils. Here, we describe a CD11b+ Siglec-F+ IL5Rα− myeloid population in the bone marrow of C57BL/6 mice. The CD11b+ Siglec-F+ IL5Rα− cells are retained in eosinophil deficient PHIL mice, and are not expanded upon overexpression of IL-5, indicating that they are upstream or independent of the eosinophil lineage. We show these cells to have GMP-like developmental potential in vitro and in vivo, and to be transcriptionally distinct from the classically described GMP population. The CD11b+ Siglec-F+ IL5Rα− population expands in the bone marrow of Myb mutant mice, which is potentially due to negative transcriptional regulation of Siglec-F by Myb. Lastly, we show that the role of Siglec-F may be, at least in part, to regulate GMP viability.
AB - In recent years multi-parameter flow cytometry has enabled identification of cells at major stages in myeloid development; from pluripotent hematopoietic stem cells, through populations with increasingly limited developmental potential (common myeloid progenitors and granulocyte-macrophage progenitors), to terminally differentiated mature cells. Myeloid progenitors are heterogeneous, and the surface markers that define transition states from progenitors to mature cells are poorly characterized. Siglec-F is a surface glycoprotein frequently used in combination with IL-5 receptor alpha (IL5Rα) for the identification of murine eosinophils. Here, we describe a CD11b+ Siglec-F+ IL5Rα− myeloid population in the bone marrow of C57BL/6 mice. The CD11b+ Siglec-F+ IL5Rα− cells are retained in eosinophil deficient PHIL mice, and are not expanded upon overexpression of IL-5, indicating that they are upstream or independent of the eosinophil lineage. We show these cells to have GMP-like developmental potential in vitro and in vivo, and to be transcriptionally distinct from the classically described GMP population. The CD11b+ Siglec-F+ IL5Rα− population expands in the bone marrow of Myb mutant mice, which is potentially due to negative transcriptional regulation of Siglec-F by Myb. Lastly, we show that the role of Siglec-F may be, at least in part, to regulate GMP viability.
KW - Myb
KW - eosinophil
KW - granulocyte
KW - hematopoiesis
KW - neutrophil
UR - http://www.scopus.com/inward/record.url?scp=85045251059&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045251059&partnerID=8YFLogxK
U2 - 10.1002/JLB.1MA1217-475R
DO - 10.1002/JLB.1MA1217-475R
M3 - Article
VL - 104
SP - 123
EP - 133
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 1
ER -