Abstract
In recent years multi-parameter flow cytometry has enabled identification of cells at major stages in myeloid development; from pluripotent hematopoietic stem cells, through populations with increasingly limited developmental potential (common myeloid progenitors and granulocyte-macrophage progenitors), to terminally differentiated mature cells. Myeloid progenitors are heterogeneous, and the surface markers that define transition states from progenitors to mature cells are poorly characterized. Siglec-F is a surface glycoprotein frequently used in combination with IL-5 receptor alpha (IL5Rα) for the identification of murine eosinophils. Here, we describe a CD11b + Siglec-F+ IL5Rα− myeloid population in the bone marrow of C57BL/6 mice. The CD11b + Siglec-F+ IL5Rα− cells are retained in eosinophil deficient PHIL mice, and are not expanded upon overexpression of IL-5, indicating that they are upstream or independent of the eosinophil lineage. We show these cells to have GMP-like developmental potential in vitro and in vivo, and to be transcriptionally distinct from the classically described GMP population. The CD11b+ Siglec-F+ IL5Rα− population expands in the bone marrow of Myb mutant mice, which is potentially due to negative transcriptional regulation of Siglec-F by Myb. Lastly, we show that the role of Siglec-F may be, at least in part, to regulate GMP viability.
Original language | English (US) |
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Pages (from-to) | 123-133 |
Number of pages | 11 |
Journal | Journal of Leukocyte Biology |
Volume | 104 |
Issue number | 1 |
DOIs | |
State | Published - Jul 2018 |
Keywords
- Myb
- eosinophil
- granulocyte
- hematopoiesis
- neutrophil
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Cell Biology