Identification of an antigenic determinant of mouse lactate dehydrogenase C4

Victoria Gonzales-Prevatt*, Thomas E. Wheat, Erwin Goldberg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A peptide bearing an antigenic determinant of the sperm-specific lactate dehydrogenase C4 isozyme (LDH-C4) has been isolated from a tryptic digest of the whole protein. This peptide, comprising residues 152-159 (MC152-159), reacts with rabbit anti-mouse LDH-C4. Immunization of rabbits with synthetic MC152-159 conjugated to bovine serum albumin induces an immune response which is specific for the peptide. Anti-MC152-159 IgG binds 125I-labeled mouse LDH-C4 and competition experiments demonstrate the specificity of this antigen-antibody reaction.

Original languageEnglish (US)
Pages (from-to)1579-1585
Number of pages7
JournalMolecular Immunology
Volume19
Issue number12
DOIs
StatePublished - Jan 1 1982

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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